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| 野菊花栓剂质量标准研究 | |
| 引用本文： | 彭朋,程雪梅,郭英,王长虹,蒋健,王峥涛.野菊花栓剂质量标准研究[J].中国实验方剂学杂志,2011,17(18):81-85. |
| 作者姓名： | 彭朋 程雪梅 郭英 王长虹 蒋健 王峥涛 |
| 作者单位： | 1. 上海中药标准化研究中心;上海中医药大学中药研究所;中药标准化教育部重点实验室;中药新资源与质量标准综合评价国家中医药管理局重点研究室,上海201203;上海中医药大学附属曙光医院临床药理科,上海201203 2. 上海中药标准化研究中心;上海中医药大学中药研究所;中药标准化教育部重点实验室;中药新资源与质量标准综合评价国家中医药管理局重点研究室,上海201203 3. 山东鲁泰环中制药有限公司,山东青岛,255086 4. 上海中医药大学附属曙光医院临床药理科,上海,201203 |
| 基金项目： | 国家药典委员会《中国药典》2010版一部标准研究基金资助(课题编号:TS-54) |
| 摘 要： | 目的:建立野菊花栓剂的质量标准。方法:木犀草苷、蒙花苷为指标,采用TLC法对野菊花栓剂进行鉴别;采用HPLC法测定野菊花栓剂中绿原酸、蒙花苷的含量。结果:在硅胶G薄层板上,以乙酸乙酯-丙酮-甲酸-水(7∶3∶1∶1)为展开剂,以2%三氯化铝乙醇溶液为显色剂,在TLC色谱中检出野菊花栓剂中的木犀草苷、蒙花苷。采用色谱柱为Agilent Eclipse,流动相为甲醇-乙腈-0.5%冰醋酸(5∶6∶89),检测波长为326 nm,栓剂中绿原酸与其他成分分离度良好。再以甲醇-0.5%冰醋酸(47∶53)为流动相,检测波长326 nm条件下,蒙花苷与其他成分具有良好的分离度。绿原酸和蒙花苷分别在3.27～408.40 mg.L-1和0.63～79.10 mg.L-1与峰面积呈良好的线性关系,平均回收率分别为100.16%,96.99%,RSD分别为1.44%,0.88%。结论:所建立的方法简便可行,重现性好,可用于野菊花栓剂的质量控制。 |
| 关 键 词： | 栓剂 野菊花 绿原酸 木犀草苷 蒙花苷 薄层色谱 高效液相色谱法 质量标准 |
| 收稿时间： | 2011/5/21 0:00:00 |
Quality Standard of Suppository for Flos Chrysanthemi Indici |
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| PENG Peng,CHENG Xue-mei,GUO Ying,WANG Chang-hong,JIANG Jian and WANG Zheng-tao.Quality Standard of Suppository for Flos Chrysanthemi Indici[J].China Journal of Experimental Traditional Medical Formulae,2011,17(18):81-85. | |
| Authors: | PENG Peng CHENG Xue-mei GUO Ying WANG Chang-hong JIANG Jian and WANG Zheng-tao |
| Institution: | Shanghai R&D Center for Standardization of Chinese Medicines, The MOE Key Laboratory for Standardization of Chinese Medicines and The SATCM Key Laboratory for New Resources and Quality Evaluation of Chinese Medicines; Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China; Department of Clinical Pharmacology, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China;Shanghai R&D Center for Standardization of Chinese Medicines, The MOE Key Laboratory for Standardization of Chinese Medicines and The SATCM Key Laboratory for New Resources and Quality Evaluation of Chinese Medicines; Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China;Shandong Lutaihuanzhong Pharmaceutical Co. Ltd., Qingdao 255086, China;Shanghai R&D Center for Standardization of Chinese Medicines, The MOE Key Laboratory for Standardization of Chinese Medicines and The SATCM Key Laboratory for New Resources and Quality Evaluation of Chinese Medicines; Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China;Department of Clinical Pharmacology, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China;Shanghai R&D Center for Standardization of Chinese Medicines, The MOE Key Laboratory for Standardization of Chinese Medicines and The SATCM Key Laboratory for New Resources and Quality Evaluation of Chinese Medicines; Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China |
| Abstract: | Objective:To establish quality control system of suppository for Flos Chrysanthemi Indici(FCI) by identification and content determination.Method:The suppository for FCI was identified by TLC with luteoloside and linarin as indexes.The content of chlorogenic acid and linarin in the suppository for FCI was determined by HPLC.Result:On the silica gel thin layer plate,developed with mixture of acetic ether-acetone-formic acid-water(7:3:1:1) and sprayed with 2% aluminum trichloride ethanol solution,luteoloside ... |
| Keywords: | suppository Flos Chrysanthemi Indici chlorogenic acid luteoloside linarin TLC HPLC quality standard |
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