均匀设计法优选坤安颗粒提取工艺

目的:优化坤安颗粒的提取工艺。方法:采用均匀设计法安排试验,以加水倍量、提取时间和提取次数为主要考察因素,以芍药苷提取率及出膏率为考察指标,多指标综合评分法优化水提取工艺;以加醇体积分数、提取次数、加液倍量和提取时间为主要考察因素,以总黄酮提取量及出膏率为考察指标,多指标综合评分法优化醇提取工艺。结果:确定水提取条件为每次加12倍水,提取2次,每次1.5 h;醇提条件为加入9.6倍量30%乙醇,提取3次,每次提取2 h。结论:该提取工艺合理、稳定、可行。

Optimal Extraction Technology of Kun' an Granules by Uniform Design

Objective: To optimize extraction process of Kun'an granules. Method: Arranged experiments with uniform design method, investigated main factors of solvent dosage,extracting time and extracting times, extract percentage and extraction rate of paeoniflorin were as index, multi-index comprehensive score was used to optimum water extraction process; investigated main factors of ethanol concentration,extracting times,ethanol volume and extracting time, extract percentage and extraction rate of total flavonoids were as index, multi-index comprehensive score was used to optimum water extraction process. Result: Optimum water extraction conditions were: extracted 2 times with 12 times the amount of water for 1.5 h each time;optimum ethanol extraction conditions were: added 9.6 times the amount of 30% ethanol, extracted 3 times for 2 h each time. Conclusion: The optimized extraction process was reasonable, stable and feasible.