| 白芍总甙对大鼠腹腔巨噬细胞化学发光的影响 |
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| 引用本文： | 梁君山,陈敏珠,徐叔云. 白芍总甙对大鼠腹腔巨噬细胞化学发光的影响[J]. 中国药理学通报, 1988, 0(4) |
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| 作者姓名： | 梁君山 陈敏珠 徐叔云 |
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| 作者单位： | 安徽医科大学临床药理研究所 合肥(梁君山，陈敏珠)，安徽医科大学临床药理研究所 合肥(徐叔云) |
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| 摘 要： | 本文采用正交设计L_(25)~((5)4)对影响大鼠腹腔巨噬细胞(PMφ)鲁米诺依赖的化学发光(LDCL)实验因素—鲁米诺浓度、细胞浓度、酵母多糖浓度、酵母多糖调理时间等进行了探讨,表明其最佳反应组合水平为:100μmol/L—5×10~6/ml—10g/L—45min大鼠腹腔Mφ在有或无白芍总甙(TGP)的条件下体外培养12h,用上述各因素的亚适水平进行LDCL测定,结果表明,TGP在0.09—2.25mg/L范围内可浓度依赖性地增加Mφ的LDCL强度,当TGP浓度增至11.25mg/L时,CL降低,量效曲线呈钟罩形趋势,提示该药对LDCL的影响有双向作用。
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| 关 键 词： | 白芍总甙 化学发光 巨噬细胞 |
EFFECTS OF TOTAL GLUCOSIDES OF PAEONY ON CHEMILUMINESCENCE OF PERITONEAL MACROPHAGES IN RATS |
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| Liang Junshan,Chen Minzhu and Xu Shuyun. EFFECTS OF TOTAL GLUCOSIDES OF PAEONY ON CHEMILUMINESCENCE OF PERITONEAL MACROPHAGES IN RATS[J]. Chinese Pharmacological Bulletin, 1988, 0(4) |
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| Authors: | Liang Junshan Chen Minzhu Xu Shuyun |
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| Abstract: | Influences of the concentrations of luminol, zymosan & cells and opsonizing time of zymosan on luminol-dependent chemiluminescence( LDCL ) of peritoneal macrophages ( PMφ) in rats were investigated by the method of orthogonal analysis. The result showed that the best levels were 100μmol/L of luminol, 10 mg/ml of zymosan, 5× 10~6/ml of cells and 45 min for opsonizing time. The LDCL of PMφ was enhanced by incubation with TGP for 12 h in a concentration-dependent manner in the range of 0.09-2.25 μg/ml of TGP, but LDCL was diminished with the concentration of 11.25 μg/ml of TGP . The concentration-effect curve seems to be bell-shaped. The data suggested that TGP had two-sided regulatory effects on the LDCL of PMφ in rats, depending on the concentration used. |
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| Keywords: | Total glucosides of paeony Chemiluminescence Macrophage |
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