高效液相色谱法测定血浆中平痛新的浓度

目的 :建立高效液相色谱法测定血浆中微量平痛新浓度的分析方法。方法 :采用HPLC法 ,色谱柱 :KromasilC18 柱 (Φ4 6mm×200mm ,5μm) ;流动相 :甲醇 -水 -三乙胺 (50∶50∶0 5) ;流速 :0 7ml/min ;检测波长 :215nm ;灵敏度 :0 04AUFS ;内标物 :奥美拉唑。结果 :血浆中平痛新浓度在10～600ng/ml范围内线性关系良好 ,相关系数r=0 9993 ;平均回收率为 (100 36±1 1) % ;平均日内和日间差异分别为4 7 %和3 8 % ;最低检测限可至1ng/ml。结论 :该法简便、稳定 ,可用于盐酸平痛新体内药代动力学与生物利用度研究。

Determination of theConcentration of Nefopam in Plasma by HPLC

OBJECTIVE： To develop a method to determine the contents of nefopam in plasma by HPLC.METHODS： RP-HPLC method， Kromasil C18 column（ Φ 4.6× 200mm， 5μ m） ； mobile phase was methenol-water-triethylamine（ 50∶ 50∶ 0.5） ； Detecting wavelength was 215nm； The flow rate was 0.7ml/min； sensitivity was 0.04AUFS； HPLC internal standard was Omeprazole.RESULTS： The calibration cure was linear in the concentrations ranged from 10ng/ml to 600ng/ml（ r=0.9 993）.The average recovery was （ 100.36± 1.1） ％ .The RSD within day was less than 4.7％ and the RSD between days was less than 3.8％ .CONCLUSION： This method is simple and reliable， which also can be applied to the study of pharmacokinetics and bioavailability of nefopam in vivo.