无精子症精液细胞学和抗精子抗体检测分析

目的:探讨精液细胞学检测在诊断无精子症中的意义,了解无精子症与抗精子抗体的关系。方法:对33例无精子症患者(研究组)进行精液细胞学检测和精浆抗精子抗体(AsAb)酶联免疫检测。结果:精液细胞学检测异常23例(69.7%),抗精子抗体阳性8例(24.2%)。选择23例生育能力正常者为对照,研究组各级精液细胞学的形态与阳性组比较差异有统计学意义(P<0.005)。抗精子抗体与阳性组比较差异有统计学意义(P<0.05)。结论:精液细胞学检测是一种较理想的检查方法。在无精症患者中是由生精阻滞所致,最常见的阻滞发生在精子细胞水平。抗精子抗体阳性IgG亚型可能与活性精子匮乏有关。

Analysis of the spermatogenic cell of semen and anti-sperm antibody in 33 infertile patients

Objection: To examine the spermatogenic cell morphology of semen and anti-sperm antibody (AsAb) in 33 infertile patients. To explore the significance of it. And to find out the relationship of infertility and anti-sperm antibody. Methods: The spermatogenic cell morphological alteration and AsAb in 33 infertile patients were detected with ELISA and compared with those 23 controls. Results: The positive rate of AsAb and spermatogenic cell morphological alteration rate infertile group is 24.4%(8/33) and 69.7%(23/33) respectively, which were notably higher than those in the control group (P<0.05). Conclusions: Observation of the spermatogenic cell morphological detection is an ideal method. In aspermous Patients the reason is the obstruction of spermatogenesis, the most common obstruction occurs in the spermatid level. The positive rate of AsAb may relate to the deficiency of active spermatozoon.