氟对体外培养的哺乳类细胞毒性试验

用体外培养的大鼠肝细胞及人胎肝、肾、肺细胞观察氟对软组织的损伤。结果表明,氟浓度在39μg/ml时对大鼠肝上皮细胞,156.2μg/ml对人胎肝、肾上皮细胞均产生明显的毒性作用。而此浓度时对人胎肺纤维母细胞未见任何形态改变。氟的非骨相损害尤其是对肝、肾的慢性损伤更应予以重视。

Study on Cytotoxicity of Fluoride in Mammal Cells Cultured in Vitro

Non-skeletal phase damage of endemic fluorosis has been noticed at present. The soft tissue and enzyme metabolism are effected by fluoride and these effects appeared before skeletal change. In this paper, in order to observe the toxic effects of fluoride and to knew the mechanism of fluoride toxication, the liver cell of rat and ceils of the liver, renal, lung of human embryo were treated by fluoride. The results indicated that the concentration of fluoride 39 ug/ml produced toxic effcts to rst liver epithelium cells. The concentration of fluoride I56.2 ug/ml induced toxic effects to liver, renal epithelium cells of human embryo. In this conce ntration, lung fibroblastic cells of human embryo haven't appeared any morphaiogical changes. The result suggested that non-skeletal damage of liver and kidney should be pay attention.