胎生蜥蜴(Lacerta vivipara Jacquin)消化道5-羟色胺免疫活性内分泌细胞的研究

目的对胎生蜥蜴（Lacerta vivipara Jacquin）消化道内五羟色胺免疫阳性内分泌细胞的分布及形态进行观察。方法采用ABC（avidin—biotin—peroxidase complex）免疫组织化学方法。结果5-羟色胺（5-HT）细胞从食管到直肠各段均有分布。细胞分布密度呈波浪式，贲门、幽门部和回肠是细胞分布密度的高峰。5-HT细胞的形态呈圆形、椭圆形、锥体形、梭形等，其中以圆形和椭圆形为主；广泛分布于上皮细胞基部、上皮细胞之间、腺泡上皮及腺泡之间。结论根据其细胞形态认为，5-HT细胞具有内、外分泌两种功能；并且比较了几种爬行动物的5-HT细胞在消化中的密度分布，认为其分布型可能与其食性、生活环境等有关。

Distribution of 5-HT Immunoreactive Endocrine Cells in the Digestive Tract of Lacerta vivipara Jacquin

Objective The distribution and morphology of 5-HT immunoreactive endocrine cells in the digestive tract of Lacerta vivipara Jacquin were investigated.Methods ABC immunohistochemical technique was applied.Results The 5-HT cells distributed throughout the digestive tract,from esophagus to rectum.The distribution density curve appeared waveform and there were three density peaks: cardiac part of the stomach,pylorus of the stomach and ileum.The 5-HT cells were round, ellipse,shuttle-like or tapering in shape,mainly round and ellipse,and distributed widely at the basal region of epithelium,between glandular epithelial cells and between acini.Conclusion In view of their morphology we would suggest that 5-HT cells have both endocrine and exocrine functions. A comparison of the distribution density of 5-HT cells in the digestive tract between sevtral few reptiles suggest that the difference of their distribution density might be related to feeding habit and surroundings as well.