化州柚提取物止咳化痰平喘作用的实验研究

目的：研究化州柚提取物的止咳化痰平喘作用。方法：采用小鼠氨水引咳法和豚鼠枸橼酸引咳法观察止咳作用；用小鼠酚红排泌法和大鼠毛细玻管排痰法观察化痰作用；用喷雾致喘法观察平喘作用。结果：化州柚提取物能明显延长引起半数小鼠咳嗽的氨水喷雾时间（EDT50）,并减少枸橼酸引起的豚鼠咳嗽次数和延长咳嗽潜伏期,能促进小鼠气管酚红的排泌和增加大鼠玻管的排痰量,对组胺和氯化乙酰胆碱混合液引起的豚鼠哮喘具有抑制作用。 结论：化州柚提取物具有明显止咳、化痰和平喘作用。

Experimental studies on antitussive,expectorant and antiasthmatic effects of extract from Citrus grandis var. tomentosa

Objective: To observe the antitussive,expectorant and antiashmatic effects of the extract from Citrus grandist var.tomentosa.Method: The cough models induced by ammonia liquor in mice and citric acid in guines pigs were used to observe the antitussive effects.The methods of phenel red execretion in mice and phlegm displacement of rats were used to investigate the expectorant effects.Bronchial asthma model induced by histamine-acetylcholine in guinea pigs was used to observe the antiasthmatic effects.Result: The extract from the title plant could inhibit the cough induced by ammonia in mice,decrease the frequency of cough induced by citric acid in guineas pig and prolong the latent period of cough.The extract also could obviously increase phenol red output of trachea in mice,promote expectoration in rats and prolong the latent period of asthma induced by histamine-acetylcholine in guinea pigs.Conclusion: The extract from C.grandis var.tomenfosa has significant antitussive,expectorant and antiasthmatic effects.