重组人TRAIL促卵巢癌细胞凋亡作用的研究

目的:分析重组人可溶性肿瘤坏死因子相关的凋亡诱导配体(TRAIL)分子诱导卵巢癌细胞的凋亡。方法:观察重组人可溶性TRAIL对卵巢癌细胞直接细胞毒作用,MTT法分析不同浓度TRAIL对卵巢癌细胞3AO、HO-8910的生长抑制率,采用流式细胞技术观察TRAIL作用于3AO后的凋亡率的变化。结果:重组人可溶性TRAIL蛋白可抑制卵巢癌细胞生长,诱导卵巢癌细胞发生凋亡;倒置显微镜下观察细胞呈现圆形固缩形态,细胞凋亡之后存活细胞减少。结论:重组人可溶性TRAIL具有明显诱导卵巢癌细胞凋亡的活性,并具有量效性及时效性。其抑制卵巢癌细胞机制可能与其促凋亡作用有关。

Induction of apoptosis by TNF-related apoptosis-inducing ligand in human ovarian cancer cells

Purpose:Our study is to find out the inhibitory action of recombinant human TNF-related apoptosis-inducing ligand(TRAIL) on ovarian cancer cell line cultured in vitro. Methods:MTT was applied to assay the inhibiting action of various concentration of TRAIL on two ovarian cancer cell lines of 3AO and HO-8910.The apoptosis rates were measured by flow cytometry. Results:The growth of human ovarian cancer cells was effectively inhibited by TRAIL. A clear dose- and time-dependent correlation between TRAIL concentration and the degree of apoptosis induction was observed with up to 43.20% apoptotic cells after 24 h of incubation with 50 ng/ml TRAIL. The cells assumed typical cell apoptosis configuration. Conclusions:TRAIL can effectively inhibit the growth of ovarian cancer cells and induce apoptosis of the cells.