抑癌基因甲基化检测结直肠癌患者的研究

目的 应用甲基化特异的聚合酶链反应(PCR)法(MSP)法分析抑癌基因高甲基化对诊疗结直肠癌的应用价值。方法 应用MPS法检测20例结直肠癌患者血清、术中癌旁组织及癌组织中的抑癌基因hLMH1、p15、p16甲基化的发生率。结果 20例结直肠癌患者术前血清及术中癌组织的hLMH1、p15、p16甲基化发生率为61％—97％；癌旁组织略低；术后1周血清中hLMH1、p15、p16与术前比较明显下降(P＜0．01)，而正常对照组抑癌基因甲基化发生率均为0。结论 MSP法的建立，直接检测结直肠癌患者的抑癌基因的高甲基化状态，有助于临床判断和动态观察治疗效果。

Clinical application of tumor suppressor genes methylation detection in colonic and rectal cancers

Objective To study the clinical application of tumor suppressor genes hyper-methylation in the diagnosis and treatment of colorectal cancers.Methods By using methylation specific PCR (MSP),the attack rate of tumor suppressor genes hLMH1,p15,p16 methylation in serum,adjacent cancerous tissue and cancerous tissue of 20 cases of colorectal cancers was detected.Results In serum before operation and cancerous tissue during operation,the attack rate of tumor suppressor genes hLMH1,p15,p16 methylation was between 61% to 97%.The rate was slightly lower in adjacent cancerous tissue.One week after operation,the rate of serum hLMH1,p15 and p16 was obviously lower than before operation (P<0.01).However,in normal control group,the rate was 0.Conclusion MSP can detect directly the tumor suppressor genes hyper-methylation state.This can be applied in clinical diagnosis anddynamic observation of curative effect.