Prevalence and genetic characterization of Toxoplasma gondii in badgers (Melogale moschata) in southern China by PCR-RFLP |
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| Institution: | 1. State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu Province 730046, PR China;2. Jiangsu Co-innovation Center for the Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University College of Veterinary Medicine, Yangzhou, Jiangsu Province 225009, PR China;3. Military Veterinary Institute, Academy of Military Medical Sciences, Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Changchun, Jilin Province 130122, PR China;1. Department of Biosciences, COMSATS Institute of Information Technology, Islamabad, Pakistan;2. Department of Bioinformatics and Biosciences, Faculty of Computing, Mohammad Ali Jinnah University, Islamabad, Pakistan;3. Department of Computer Science, Faculty of Computing, Mohammad Ali Jinnah University, Islamabad, Pakistan;1. Department of Marine Biological Science and Technology, College of Ocean and Earth Sciences, Xiamen University, Xiamen 361006, China;2. Hainan Provincial Key Laboratory of Tropical Maricultural Technologies, Hainan Academy of Ocean and Fisheries Sciences, Haikou 571126, China;3. Department of Marine Sciences, College of Marine Sciences, Hainan University, Haikou 570228, China;4. State-Province Joint Engineering Laboratory of Marine Bioproducts and Technology, Xiamen University, China;5. Zhangzhou Aquatic Technology Promotion Station, Zhangzhou 363000, China;2. Faculty of Mathematics and Computer Science, Friedrich Schiller University Jena, Jena, Germany;3. FLI Leibniz Institute for Age Research, Jena, Germany |
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| Abstract: | Toxoplasma gondii is an obligate intracellular apicomplexan parasite which is able to infect almost all warm-blooded animals. There is no information about the prevalence and genetic characterization of T. gondii in badgers (Melogale moschata) in China. Here, a total of 367 badgers were captured from different cities in Jiangxi province, Southern China. Genomic DNA was extracted from brain tissues of each badgers, and 57 (15.45%) of them were positive for T. gondii by semi-nested PCR of the B1 gene. The positive DNA samples were typed at 11 genetic markers, including 10 nuclear loci (SAG1, 5′-SAG2 and 3′-SAG2, alternative SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1) and an apicoplast locus (Apico), with multilocus polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technology. Among them, 4 were completely typed at all loci, and 2 was genotyped for 9 loci, showing that they belong to ToxoDB#9. This is the first report of prevalence and genetic characterization of T. gondii isolates from badgers in China, which contributes to broader understanding of population structure of T. gondii in China. It is important for the prevention and control of T. gondii infection in wild animals. |
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