Tripterygium wilfordii polyglycosidium ameliorates pouchitis induced by dextran sulfate sodium in rats |
| |
| Affiliation: | 1. Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai, Japan;2. Graduate School of Medicine, Tohoku University, Sendai, Japan;3. Pharmaceuticals and Medical Devices Agency (PMDA), Tokyo, Japan;4. National Institute of Health Sciences (NIHS), Tokyo, Japan;5. Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama, Japan;1. Division of Gastroenterology, Stanford University School of Medicine, Stanford, California;3. Division of Colorectal Surgery, Stanford University School of Medicine, Stanford, California;6. Department of Pathology, Stanford University School of Medicine, Stanford, California;5. Surgery-Colorectal Surgery, Stanford University School of Medicine, Stanford, California;4. Division of Gastroenterology, Santa Clara Valley Medical Center, San Jose, California;1. Division of Nephrology, St. Michael''s Hospital, University of Toronto, Toronto, Ontario, Canada;2. The Keenan Research Centre in the Li Ka Shing Knowledge Institute, St. Michael''s Hospital, University of Toronto, Toronto, Ontario, Canada;3. Institute for Clinical Evaluative Sciences, Toronto, Ontario, Canada;4. Department of Medicine, Sunnybrook Hospital, University of Toronto, Toronto, Ontario, Canada;5. Division of Nephrology, London Health Sciences Centre, Western University, London, Ontario, Canada;1. Department of Institute for Control of Chinese Traditional Medicine and Ethnic Medicine, National Institutes for Food and Drug Control, Beijing 100050, China;2. Huaan Magnech Bio-Tech Co., Ltd, Beijing 102200, China;1. Division of Gastroenterology and Hepatology, Johns Hopkins Medicine, Johns Hopkins Medical Institutions, 4940 Eastern Avenue, A Building, 5th Floor, Baltimore, MD 21224, USA;2. Endoscopy Division, National Cancer Center Hospital, 5-1-1, Tsukiji, Chuo-ku, Tokyo 104-0045, Japan |
| |
| Abstract: | BackgroundThe aim of this study was to investigate the therapeutic effects of Tripterygium wilfordii polyglycosidium (TWP) to rats with dextran sulfate sodium (DSS)-induced pouchitis and its possible mechanism.MethodsSprague-Dawley rats underwent surgery of ileal pouch anal anastomosis (IPAA) and pouchitis was induced by DSS. Rats were randomly divided into no intervention (NI), normal saline (NS) and TWP groups. Rats were lavaged with normal saline (3 ml/day in NS group) or TWP (12 mg/kg/day in TWP group) for 7 days. General conditions of animals and histopathological examinations were evaluated. Interleukin (IL)-1β, IL-6, IL-10, and tumor necrosis factor (TNF)-α mRNA expression was measured. Levels of occludin and Zo-1 proteins were measured by immunohistochemistry. In addition, ALT and AST were assessed.ResultsTWP significantly attenuated the symptoms of pouchitis characterized by body weight loss, diarrhea, and bloody stool. Furthermore, TWP diminished histological damage compared with other groups. There was a significant reduction in levels of IL-1β, IL-6, and TNF-α, as well as an increase in IL-10 in the TWP group. The expression of tight junction proteins occludin and Zo-1 were increased in the TWP group. There were no statistical differences in serum ALT and AST among the three groups.ConclusionsTWP significantly ameliorated pouchitis and inhibited the production of IL-1β, IL-6, and TNF-α as well as increased the levels of IL-10, occludin, and Zo-1 protein in rats. These findings suggest TWP might be a potential therapeutic agent for patients with pouchitis. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
