Claudin-1及E-cadherin与乳腺癌关系的研究

目的:探讨紧密连接蛋白1(claudin-1,Cldn-1)和上皮钙黏蛋白(E-cadherin,E-cad)在乳腺癌组织中的表达及其与乳腺癌侵袭转移的关系。方法:应用组织芯片技术和免疫组化法检测77例乳腺癌标本中Cldn-1和E-cad的表达情况,并分析两者与乳腺癌淋巴结转移、TNM分期和肿块最大径以及组织学分级间的关系。结果:Cldn-1和E-cad表达强度与乳腺癌的淋巴结转移及临床分期有关,有淋巴结转移的乳腺癌组Cldn-1和E-cad表达强度明显弱于无淋巴结转移组(P=0.0009,P=0.014);Cldn-1在TNMIII期的表达强度分别弱于I期和II期(P=0.046,P=0.047)。而E-cad的表达强度II期及III期均弱于I期(P=0.021,P=0.001)。Cldn-1和E-cad表达强度与肿块最大径各分组间及组织学各分级之间差异无统计学意义(P=0.967,P=0.150及P=0.188,P=0.164)。Cldn-1与E-cad两者在乳腺癌组织中的表达强度差异无统计学意义(P=0.747)。结论:在乳腺癌的发展和转移中Cldn-1及E-cad的表达强度均呈现减弱趋势,但两者可能是独立的因素;联合检测有助于对乳腺癌高复发危险性的筛选,两者可作为判断乳腺癌淋巴结转移、复发和评估预后的参考指标。

Relations of claudin-1 and E-cadherin with breast cancer

Objective:To investigate the expressions of tight junction protein claudin-1(Cldn-1) and E-cadherin(E-cad) in breast cancer tissue and their relations with invasion and metastasis of this cancer. Methods:The expressions of Cldn-1 and E-cad in the breast cancer samples(n=77) were detected by using tissue chip technology and immunohitochemisty.The relations of Cldn-1 and E-cad with lymph node metastases,TNM stage,maximum lump diameter and histological classification of breast cancer were analyzed. Results:The expression intensities of Cldn-1 and E-cad were associated with lymph node metastases and clinical stage of breast cancer.The results showed that the expression intensities of Cldn-1 and E-cad in the breast cancer tissues with nodal metastases were significantly lower than those without lymph node metastases(P=0.0009,P=0.014),the expression intensity of Cldn-1 in stage III breast cancer was significantly lower than that in stage I and stage II(P=0.046,P=0.047),and the expression intensities of E-cad in both stage II and stage III breast cancer were significantly lower than that in stage I(P=0.021,P=0.001).The expression intensities of Cldn-1 and E-cad were unrelated to the maximum lump diameter and histological classification of the tumor(P=0.967,P=0.150 and P=0.188,P=0.164).There was no significant difference between the expression intensities of Cldn-1 and E-cad in breast cancer(P=0.747). Conclusion:Both Cldn-1 and E-cad expressions show a decreasing trend in development and metastases of breast cancer,but both may be independent factors.Combination measurement of Cldn-1 and E-cad is of value for the screening of breast cancer with high risk of recurrence,and they may be potential indicators for metastases,recurrence and prognostic estimation of breast cancer.