| C-myc单克隆抗体的制备及鉴定 |
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| 引用本文： | 孙慧慧,王旭,王惠国,谭建华,朴敬爱,朴君,马彪,刘庆平,李文哲.C-myc单克隆抗体的制备及鉴定[J].免疫学杂志,2011(8):688-691. |
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| 作者姓名： | 孙慧慧 王旭 王惠国 谭建华 朴敬爱 朴君 马彪 刘庆平 李文哲 |
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| 作者单位： | 辽宁师范大学生命科学学院;大连大学生物工程学院免疫研究所;军事医学科学院军事兽医研究所; |
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| 基金项目： | 国家自然科学基金(30972675); 大连市科技计划项目(2010J21DW011); 国家教育部归国留学人员科研启动资金[教外司留(2008)101] |
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| 摘 要： | 目的制备与鉴定鼠抗C-myc蛋白单克隆抗体。方法利用C-myc蛋白做免疫原免疫Balb/c小鼠,取免疫小鼠脾细胞与SP2/0骨髓瘤细胞进行细胞融合,经间接ELISA法筛选、有限稀释法克隆、单克隆抗体Ig亚型鉴定,获得2株能够稳定分泌抗C-myc特异性抗体的杂交瘤细胞株,再将杂交瘤细胞注射入小鼠腹腔诱使小鼠产生腹水。结果获得2株可稳定分泌抗C-myc抗体的杂交瘤细胞株,命名为SHH-1H11,SHH-2A9,Ig亚型分别为IgG1亚类和IgM。细胞培养上清效价分别为1:10240、1:5 120;小鼠腹水效价分别为1:64 000、1:32 000。结论制备的C-myc单克隆抗体仅与C-myc蛋白反应,与其他蛋白没有交叉反应,表明获得的单克隆抗体的特异性很高。
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| 关 键 词： | C-myc 单克隆抗体 制备 鉴定 |
Preparation and identification of monoclonal antibodies against C-myc |
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| SUN Huihui,WANG Xu,WANG Huiguo,TAN Jianhua,PIAO Jingai,PIAO Jun,MA Biao,LIU Qingping,LI WenzheSchool of Life Science,Liaoning Normal University,Liaoning,Chin.Preparation and identification of monoclonal antibodies against C-myc[J].Immunological Journal,2011(8):688-691. |
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| Authors: | SUN Huihui WANG Xu WANG Huiguo TAN Jianhua PIAO Jingai PIAO Jun MA Biao LIU Qingping LI WenzheSchool of Life Science Liaoning Normal University Liaoning Chin |
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| Institution: | SUN Huihui,WANG Xu,WANG Huiguo,TAN Jianhua,PIAO Jingai,PIAO Jun,MA Biao,LIU Qingping,LI WenzheSchool of Life Science,Liaoning Normal University,Liaoning,116029,Chin |
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| Abstract: | To prepare monoclonal antibodies against for C-myc protein,Balb/c mice were immunized with C-myc protein and the splenocytes of the immunized mice were fused with the SP2/0 myeloma cells by hybridoma technique.Two hybridoma cell lines secreting monoclonal antibodies against C-myc were obtained,which were determined as SHH-1H11,SHH-2A9.The subtypes of antibodies,SHH-1H11 and SHH-2A9 were IgG1 and IgM,respectively.The ELISA titer of antibodies produced by SHH-1H11 and SHH-2A9 were 1:10 240 and 1:5 120 in cell... |
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| Keywords: | C-myc Monoclonal antibody Preparation Hybridoma |
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