| SEA-CD80基因过表达Hepa1-6肝癌细胞体外诱导的免疫学效应 |
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| 引用本文： | 司少艳,史亮,刘俊丽,宋淑军,景青萍,吴继华,张建中.SEA-CD80基因过表达Hepa1-6肝癌细胞体外诱导的免疫学效应[J].免疫学杂志,2011(9):747-751. |
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| 作者姓名： | 司少艳 史亮 刘俊丽 宋淑军 景青萍 吴继华 张建中 |
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| 作者单位： | 解放军第306医院病理实验科;解放军第306医院口腔科; |
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| 摘 要： | 目的观察经SEA和CD80重组腺病毒感染Hepa1-6肝癌细胞后体外能否诱导抗肿瘤免疫学反应。方法 Hepa1-6细胞分别经空载体腺病毒Ad(空)和重组腺病毒Ad-MMRE-mTERT-B7、Ad-MMRE-mTERT-SEA、Ad-MMRE-mTERT-BIS感染后,和小鼠脾淋巴细胞共培养,然后采用Brdu酶联免疫法(ELISA)检测淋巴细胞增殖;流式细胞术检测T淋巴细胞亚群增殖;ELISA法检测细胞因子IL-2、TNF-α和IFN-γ的产生;LDH释放法检测CTLs对Hepa1-6的杀伤作用。结果与感染空载体腺病毒Ad(空)和未感染Hepa1-6细胞相比,经重组腺病毒感染的Hepa1-6细胞体外能够显著诱导脾淋巴细胞的增殖,其中CD3+CD4+和CD3+CD8+T淋巴细胞增殖显著;细胞因子TNF-α、IFN-γ和IL-2的产生显著升高;CTLs对肿瘤细胞的杀伤活性显著增强;双基因过表达Hepa1-6细胞体外诱导的免疫应答高于单基因过表达Hepa-6细胞。结论 Hepa-6细胞经重组腺病毒感染后,表达在细胞膜上的SEA和CD80均具有免疫学活性,为今后采用所构建重组腺病毒进行肿瘤的免疫基因治疗提供了实验依据。
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| 关 键 词： | 金黄色葡萄球菌肠毒素A CD80 腺病毒 体外 抗肿瘤免疫 |
Immune responses induced by Hepa1-6 hepotoma cell after over-expression SEA and CD80 in vitro |
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| SI Shaoyan,SHI Liang,LIU Junli,SONG Shujun,JING Qingping,WU Jihua,ZHANG Jianzhong.Immune responses induced by Hepa1-6 hepotoma cell after over-expression SEA and CD80 in vitro[J].Immunological Journal,2011(9):747-751. |
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| Authors: | SI Shaoyan SHI Liang LIU Junli SONG Shujun JING Qingping WU Jihua ZHANG Jianzhong |
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| Institution: | SI Shaoyan,SHI Liang,LIU Junli,SONG Shujun,JING Qingping,WU Jihua,ZHANG Jianzhong Department of Pathology and Experimental Medicine,306th Hospital of PLA,Beijing,100101,China |
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| Abstract: | This study is aimed to determine whether Hepa1-6 hepotoma cells could induce antitumor immune response after over-expression Staphylococcus enterotoxin A(SEA) and CD80.Mouse spleen lymphocytes were cocultured with hepa1-6 cells infected with empty adenoviruse vector Ad(empty) or the recombinant adenoviruses of Ad-MMRE-mTERT-B7,Ad-MMRE-mTERT-SEA or Ad-MMRE-mTERT-BIS.Lymphocyte proliferation was assayed by BrdU incorporation using a commercial kit,flow cytometric analysis was used to detect proliferation of T... |
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| Keywords: | Staphylococcus enterotoxin A CD80 Adenovirus in vitro Antitumour immunity |
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