| TgAb时间分辨荧光免疫分析间接测定法的建立及临床应用 |
| |
| 引用本文： | 朱岚,黄飚,李跃松,张珏,王柯,陈永伟. TgAb时间分辨荧光免疫分析间接测定法的建立及临床应用[J]. 免疫学杂志, 2011, 0(8): 715-718 |
| |
| 作者姓名： | 朱岚 黄飚 李跃松 张珏 王柯 陈永伟 |
| |
| 作者单位： | 江苏省原子医学研究所卫生部核医学重点实验室; |
| |
| 摘 要： | 目的建立甲状腺球蛋白抗体(TgAb)时间分辨荧光免疫分析(TRFIA)间接检测法。方法用Tg抗原包板,用铕(Eu3+)标记兔抗人IgG做标记物,间接法TRFIA检测人血清中的TgAb。结果 TgAb-TRFIA的灵敏度为1.0 IU/ml;批内变异系数(CV)为3.1%～3.6%,批间CV为3.3%～3.6%;平均回收率为101.6%;热稳定性好;与电化学发光分析技术(ECLIA)比对,相关系数达0.8945;与临床结果高度相关。结论本法建立的TgAb-TRFIA是一个高灵敏和可靠的检测,有助于甲状腺疾病的临床诊断。
|
| 关 键 词： | 甲状腺球蛋白抗体 时间分辨荧光免疫分析 间接法 |
Establishment of an indirect TRFIA for thyrogloblin antibody and its clinical application |
| |
| ZHU Lan,HUANG Biao,LI Yuesong,ZHANG Jue,WANG Ke,CHEN YongweiJiangsu Institute of Nuclear Medicine,Wuxi ,Chin. Establishment of an indirect TRFIA for thyrogloblin antibody and its clinical application[J]. Immunological Journal, 2011, 0(8): 715-718 |
| |
| Authors: | ZHU Lan HUANG Biao LI Yuesong ZHANG Jue WANG Ke CHEN YongweiJiangsu Institute of Nuclear Medicine Wuxi Chin |
| |
| Affiliation: | ZHU Lan,HUANG Biao,LI Yuesong,ZHANG Jue,WANG Ke,CHEN YongweiJiangsu Institute of Nuclear Medicine,Wuxi 214063,Chin |
| |
| Abstract: | An indirect time-resolved fluoroimmunoassay(TRFIA) for thyrogloblin antibody(TgAb) was established and its clinical application was evaluated.The Tg antigen was coated on the 96 well plates.A rabbit anti-human IgG-Eu3+ was used to enable detection.Serum TgAb level was detected with the kit by TRFIA.Assay sensitivity was 1.0 IU/ml.Intrassay coefficient of variation was 3.1%~3.6%.Interassay coefficient of variation was 3.3%~3.6%.Average recovery was 101.6%.The kit was stable.Correlation coefficient with TgAb ... |
| |
| Keywords: | Thyrogloblin antibody Time-resolved fluoroimmunoassay Indirect TRFIA |
| 本文献已被 CNKI 等数据库收录！ |
|
