| 辛伐他汀对幼鼠骨小梁中骨形成相关因子表达以及BMSCs成骨分化的影响 |
| |
| 引用本文： | 张柳,刘晓宁,田发明,张辉,韩大成. 辛伐他汀对幼鼠骨小梁中骨形成相关因子表达以及BMSCs成骨分化的影响[J]. 中国修复重建外科杂志, 2011, 0(2): 155-159 |
| |
| 作者姓名： | 张柳 刘晓宁 田发明 张辉 韩大成 |
| |
| 作者单位： | 华北煤炭医学院附属医院骨外科;河北医科大学研究生院; |
| |
| 基金项目： | 河北省自然科学基金资助项目(C2006000580)~~ |
| |
| 摘 要： | 目的既往研究表明辛伐他汀具有促成骨作用,现探讨辛伐他汀对幼鼠骨小梁中骨形成相关因子表达以及BMSCs成骨分化的影响。方法 40只1周龄雄性SD大鼠,体重17.5～19.5 g,随机分为2组,每组20只。实验组于大鼠颈部皮下注射辛伐他汀注射液[5 mg/(kg.d)],对照组同法注射等剂量生理盐水。于注射后1、3周,两组分别脱颈处死10只大鼠,采用免疫组织化学染色检测胫骨上端骨小梁BMP-2、基质金属蛋白酶13(matrix metalloproteinase 13,MMP-13)、VEGF的表达情况。注射后1、3周,两组分别取大鼠双侧股骨,采用全骨髓培养法体外分离培养BMSCs,取第1代BMSCs成骨诱导培养,培养后14 d行ALP染色,培养后21 d采用实时荧光定量PCR检测BMP-2、Runx2、Osterix、Msx2、Dlx3、Dlx5 mRNA的表达,培养后28 d行von Kossa染色。结果免疫组织化学染色示注射后1、3周两组胫骨上端骨小梁BMP-2、MMP-13、VEGF表达,差异均无统计学意义(P>0.05)。成骨诱导培养14 d,注射后1、3周两组ALP染色阳性细胞百分比差异均无统计学意义(P>0.05);成骨诱导培养21 d,实时荧光定量PCR检测示注射后1、3周两组BMP-2、Runx2、Osterix、Dlx3、Dlx5、Msx2 mRNA表达水平比较差异均无统计学意义(P>0.05);成骨诱导培养28 d,注射后1、3周两组钙结节单位面积比差异均无统计学意义(P>0.05)。结论皮下注射辛伐他汀5 mg/(kg.d)后1、3周对幼鼠骨小梁中骨形成相关因子表达以及BMSCs成骨分化无明显影响。
|
| 关 键 词： | 辛伐他汀 BMSCs 成骨分化 骨形成相关因子 大鼠 |
EFFECTS OF SIMVASTATIN ON BONE FORMATION RELATIVE FACTORS OF TRABECULAR BONE AND OSTEOGENIC DIFFERENTIATION OF BONE MARROW MESENCHYMAL STEM CELLS IN YOUNG RATS |
| |
| ZHANG Liu,LIU Xiaoning,TIAN Faming,ZHANG Hui,HAN Dacheng. EFFECTS OF SIMVASTATIN ON BONE FORMATION RELATIVE FACTORS OF TRABECULAR BONE AND OSTEOGENIC DIFFERENTIATION OF BONE MARROW MESENCHYMAL STEM CELLS IN YOUNG RATS[J]. Chinese journal of reparative and reconstructive surgery, 2011, 0(2): 155-159 |
| |
| Authors: | ZHANG Liu LIU Xiaoning TIAN Faming ZHANG Hui HAN Dacheng |
| |
| Affiliation: | ZHANG Liu1,LIU Xiaoning1,TIAN Faming2,ZHANG Hui1,HAN Dacheng1.1Department of Orthopedics,Affiliated Hospital of North China Coal Medical University,Tangshan Hebei,063000,P.R.China,2Graduate School of Hebei Medical University. |
| |
| Abstract: | Objective Simvastatin has been reported to be effective on stimulation of bone formation.To investigate the effects of simvastatin on bone formation relative factors of proximal tibia trabecular bone and on osteogenic differentiation of bone marrow mesenchymal stem cells(BMSCs).Methods Fourty 1-week-old male Sprague Dawley rats were divided randomly into 2 groups,20 rats per group.Rats in experimental group received subcutaneous injection of simvastatin [(5 mg/(kgo d)],and the rats in control group received... |
| |
| Keywords: | Simvastatin Bone marrow mesenchymal stem cells Osteogenic differentiation Bone formation relative factors Rat |
| 本文献已被 CNKI 等数据库收录！ |
|
