初发点滴型银屑病患者皮损表面的微生物菌群结构发生变化

目的探究初发点滴型银屑病患者与正常人群皮肤表面微生物菌群结构的差异。方法纳入18例初发点滴型银屑病患者,其中9例具有上呼吸道感染症状(银屑病上感组),9例不具有上呼吸道感染症状(银屑病非上感组),以9例同年龄段正常人作为对照组。收集上述人群皮损和皮肤表面样本,采用16S rDNA测序技术对扩增后样本DNA测序,对测序结果进行注释,将实验数据进行生物信息学、医学统计学分析,分析三组间差异性微生物物种。结果在门水平,三组间优势物种均为厚壁菌门、变形菌门、放线菌门。三组间α多样性分析差异无统计学意义(均P>0.05)。β多样性分析,对照组与银屑病上感组、非上感组组间生物群落构成差异均有统计学意义(均P<0.05)。银屑病非上感组γ-变形菌纲、丙酸杆菌属低于正常组(均P<0.05);银屑病上感组劳森菌属、放线菌属低于正常组(均P<0.05),而未明确定义周蝶菌科丰度较高(P=0.011);银屑病上感组γ-变形菌纲、栖水菌属、假单胞菌属丰度高于银屑病非上感组(均P<0.05)。结论初发点滴型银屑病有上感者、无上感者及正常人群三者间皮肤表面微生物组成结构在属水平均存在差异。

Analysis of microbial flora in skin lesions of primary acute guttate psoriasis

Objective To investigate the differences in the cutaneous microbiome between patients with primary acute guttate psoriasis and healthy controls. Methods We enrolled 18 patients with primary acute guttate psoriasis, including 9 patients with upper respiratory tract infection and 9 patients without upper respiratory tract infection,and 9 healthy controls. Skin swabs were collected from lesional and non-lesional skin of psoriatic patients and healthy controls. The 16S rDNA sequencing was used for analyzing the DNA samples and annotating the sequencing results. The differences in microbial species and community structure among the three groups were determined by bioinformatics and statistics analysis. Results At the Phylum level, the dominant species among the three groups were Firmicutes, Proteobacteria and Actinobacteria. There was no significant difference in α diversity analysis among the three groups (all P>0.05). There were significant differences in the composition of microbial community between the control group and the two groups of patients (both P<0.05). Gammaproteobacteria and Cutibacterium in the primary acute guttate psoriasis without upper respiratory tract infection were lower than those in the normal group(both P<0.05).Campylobacteria, Lawsonella and Actinomyces in the primary acute guttate psoriasis with upper respiratory tract infection were lower than those in the normal group(both P<0.05), but unidentified Weeksellaceae was the opposite (P<0.05). Gammaproteobacteria, Enhydrobacter and Pseudomonas in the primary acute guttate psoriasis without upper respiratory tract infection were lower than that in primary acute guttate psoriasis with upper respiratory tract infection(all P<0.05). Conclusions There are differences in the composition of microbial community among the three groups at the genus level. Upper respiratory tract infection affects the composition of microflora on the skin lesion of acute guttate psoriasis. The pathogenesis of psoriasis is associated with cutaneous microbiome.