灯盏花素调控急性肺栓塞大鼠右心室CINC-1、CINC-2的mRNA及蛋白水平

  目的  观察灯盏花素对大鼠肺栓塞模型右心室CINC-1、CINC-2的mRNA及蛋白水平有无影响。   方法  复制大鼠肺栓塞模型，灯盏花素低、高剂量组及低分子肝素组给予腹腔注射相应药物，取肺组织做肺栓塞病理检测，右心室组织做Q-PCR及Western blot测定各组CINC-1、CINC-2的基因及蛋白水平。   结果  与假手术组比较，模型组CINC-1、CINC-2的基因表达明显升高（P < 0.01），灯盏花素低、高剂量组及低分子肝素组可以显著下调CINC-1、CINC-2的基因水平（P < 0.01），灯盏花素高剂量组在下调CINC-1基因书水平上优于低分子肝素组（P < 0.05）；与假手术组比较，模型组CINC-1、CINC-2的蛋白表达明显升高（P < 0.01），灯盏花素低、高剂量组可以明显下调CINC-1的蛋白水平（P < 0.01），灯盏花素高剂量组在下调CINC-1蛋白表达水平上优于低分子肝素组（P < 0.05）；灯盏花素高剂量组可以明显下调CINC-2的蛋白水平（P < 0.01）。  结论  灯盏花素可以下调急性肺栓塞大鼠右心室 CINC-1、CINC-2 的基因和蛋白表达水平， 可能发挥抗肺栓塞炎症的作用。

Breviscapine Regulates the mRNA and Protein Levels of CINC-1 and CINC-2 in the Right Ventricle of Rats with Acute Pulmonary Embolism

  Objective  To observe whether breviscapine has any effect on the mRNA and protein levels of CINC-1 and CINC-2 in the right ventricle of rats with pulmonary embolism.   Methods  The rat model of pulmonary embolism was replicated, and rats in the breviscapine low-dose, high-dose and low-molecular-weight heparin groups were given intraperitoneal injection of corresponding drugs, lung tissue samples were collected for pathological detection of pulmonary embolism, and right ventricular tissue samples were determined by Q-PCR and Western blot to determine CINC-1, CINC-2 gene and protein levels in each group.   Results  Compared with the sham operation group, the gene expression of CINC-1 and CINC-2 in the model group was significantly upregulated （P < 0.01）. The low-dose, high-dose and low-molecular-weight heparin groups of breviscapine could significantly down-regulate the gene expression of CINC-1 and CINC-2 （P < 0.01）. The high-dose breviscapine group was better than the low-molecular-weight heparin group in down-regulating CINC-1 gene level （P < 0.05）. Compared with the sham operation group, the protein expressions of CINC-1 and CINC-2 in the model group were significantly increased （P < 0.01）. The low-dose and high-dose groups of breviscapine could significantly down-regulate the protein level of CINC-1 （P < 0.01）, and the high-dose group of breviscapine was better than the low-molecular-weight heparin group in down-regulating the protein expression of CINC-1 （P < 0.05）. And the high dose of breviscapine can significantly down-regulate the protein level of CINC-2 （P < 0.01）.   Conclusion  Breviscapine may play an anti-inflammatory role in pulmonary embolism by down-regulating the gene and protein expression of CINC-1 and CINC-2 in the right ventricle of rats with acute pulmonary embolism.