不同富集培养方法对噬菌体PEf771的滴度影响

目的 对比3种不同富集培养方法对噬菌体PEf771的滴度影响,探讨有效提高噬菌体PEf771滴度的方法.方法 通过噬菌体PEf771/粪肠球菌E.faecalis YN771比例法、挖斑法和丝裂霉素C诱导法在E.faecalis YN771不同生长时期加入PEf771原液进行富集,来获得较高的PEf771的滴度.结果 ...

Effects of Different Accumulation Culture Methods on the Titer of Phage PEf771

  Objective  To compare the effects of three different enriching culture methods on the titer of phage PEf771, and discuss the methods to effectively improve the titer of phage PEf771.   Methods  Through phage PEf771/E. faecalis YN771 proportional method, spot digging method and mitomycin C induction method, phage PEf771 stock solution was added at E. faecalis YN771 different growth stages for enrichment to obtain a higher titer of phage PEf771.   Results  The titer of PEf771 obtained by PEf771/E. faecalis YN771 = 1∶3 and spot digging method was higher, there was no significant difference between the two methods after expanded culture （P > 0.05）, and the titer reached 108 pfu/mL in 24 hours. Mitomycin C induction method could not significantly improve the titer of PEf771.PEf771 was added in the early growth stage （OD600 = 0.3） and logarithmic stage （OD600 = 1） of E. faecalis YN771 for enrichment culture. The titer of the former was 1010 pfu/mL after 6 hours of culture, and remained 1010 pfu/mL after 24 hours, reaching the ideal titer.   Conclusion  Adding PEf771 in the early stage of E. faecalis YN771 culture （OD600 = 0.3） and enriching culture with PEf771/E. faecalis YN771 = 1∶3 or spot digging method can effectively improve the titer of PEf771. It provides experimental materials and basis for the study of phage PEf771 of E. faecalis YN771 in the treatment of infection caused by E. faecalis YN771.