肝细胞癌癌组织caspase-3和DcR3蛋白及SNHG12 mRNA水平变化及其临床意义探讨*

目的 研究肝细胞癌(HCC)患者癌组织半胱氨酰天冬氨酸特异性蛋白酶-3(caspase-3)和诱捕受体3(DcR3)蛋白表达及小分子核仁宿主基因12(SNHG12)mRNA水平变化及其临床意义。方法 2016年5月～2019年5月我院收治的68例HCC患者,行肝叶切除术治疗,取癌组织和癌旁肝组织,采用EnVision法检测caspase-3和DcR3蛋白表达,采用RT-qPCR法检测SNHG12 mRNA水平,应用Logistic多元回归分析影响HCC患者死亡的危险因素。结果 HCC患者癌组织caspase-3阳性率为47.1%,显著低于癌旁组织的77.9%(P＜0.05),而DcR3阳性率为61.8%,SNHG12 mRNA水平为(2.9±0.8),显著高于癌旁组织【分别为33.8%和(1.6±0.5),P＜0.05】;18例有淋巴结转移的癌组织caspase-3阳性率为33.3%,显著低于50例无淋巴结转移者的62.0%(P＜0.05),而DcR3蛋白阳性率为88.9%,显著高于无淋巴结转移者的50.0%,SNHG12 mRNA水平为(2.6±0.4),显著高于无淋巴结转移者【(1.5±0.3),P＜0.05】,45例肿瘤直径>5 cm癌组织SNHG12 mRNA水平为(2.7±0.4),显著高于23例无淋巴结转移者【(1.4±0.3),P＜0.05】;随访结束时,本组68例HCC患者生存30例(44.1%),死亡38例(55.9%);多元Logistic回归分析显示,肿瘤直径大、合并肝硬化、存在淋巴结转移、Caspase-3低表达、DcR3高表达和SNHG12高水平是HCC患者死亡的危险因素。结论 HCC患者癌组织存在基因水平和蛋白表达的显著变化,了解这些变化并探讨其临床意义,将为肝癌防治提供有价值的线索。

Caspase-3, DcR3 expression and SNHG12 mRNA level in cancerous tissues in patients with hepatocellular carcinoma

Objective The aim of this study was to explore the implication of cysteinyl aspartate specific protease-3(caspase-3), decoy receptor 3 (DcR3) expression and small nucleolar host gene 12 (SNHG12) mRNA level in cancerous tissues of patients with hepatocellular carcinoma (HCC). Methods 68 patients with HCC were enrolled in our hospital between May 2016 and May 2019, and all underwent hepatectomy. The expressions of caspase-3 and DcR3 proteins in cancerous and adjacent liver tissues were detected by EnVision, and the SNHG12 mRNA level was assayed by RT-qPCR. The risk factors impacting prognosis of patients with HCC was analyzed by multivariate Logistic regression. Results The positive rate of caspase-3 in cancerous tissues of patients with HCC was significantly lower than that in adjacent liver tissues (47.1% vs. 77.9%, P<0.05), while the positive rate of DcR3 was 61.8%, the SNHG12 mRNA load was (2.9±0.8), significantly higher than 33.8% and (1.6±0.5), P<0.05] in adjacent tissues; the cancerous caspase-3 positive rate in 18 patients with lymph node metastasis was 33.3%, significantly lower than 62.0%(P＜0.05)in 50 patients without, while the DcR3 positive rate was 88.9%, significantly higher than 50.0% in patients without, the SNHG12 mRNA load was (2.6±0.4), significantly higher than (1.5±0.3), P＜0.05] in patients without, and the SNHG12 mRNA level in 45 patients with greater than 5 cm tumor was (2.7±0.4), significantly higher than (1.4±0.3), P＜0.05] in 23 patients with tumor less than 5 cm; at the end of follow-up, 30(44.1%) patients survived and 38 patients(55.9%) died; the multivariate Logistic regression analysis showed that large tumor mass, with underlying liver cirrhosis, lymph node metastasis, with caspase-3 weak expression, DcR3 strong expression and high SNHG12 mRNA load were the risk factors impacting the prognosis of patients with HCC after hepatectomy. Conclusion The down-regulated expression of caspase-3 and the intensified expression of DcR3 protein in cancerous tissues of patients with HCC hint the carcinogenesis, which might provide some clues for the prevention and management of the tumor.