不同产地苍艾挥发油的GC-MS指纹图谱研究

目的:建立苍艾挥发油的气相色谱-质谱(GC-MS)指纹图谱,分析苍艾挥发油的化学组成,为其质量控制提供方法和依据.方法:Agilent DB-5MS石英毛细管色谱柱(30m×0.25mm×0.25μm),柱温40℃(保持1 min),以10℃·min-1速率升至130℃(保持5 min),以8℃·min-1速率升至25...

Study on GC-MS fingerprints of Cangai volatile oil from different origins

OBJECTIVE To establish the GC-MS fingerprints and analyze the chemical components of Cangai volatile oil from different origins in order to provide the method and basis for its quality control.METHODS The analysis was achieved by using an Agilent DB-5 MS(30 m×250 mm×0.25 μm) silica capillary chromatographic column(40 ℃ for 1 min, 10 ℃·min^-1 to 130 ℃ for 5 min, 8 ℃·min^-1 to 250 ℃ for 5 min). The injector temperature was 280 ℃ and the carrier gas was high purity helium at the flow rate of 1.0 mL·min^-1, the shunt ratio was 40∶1 and the injection volume was 1 μL. The total separation time was 35 min and solvent delay time was 4 min. MS conditions were as follows: electron impact(EI) ion source, ion source temperature of 230 ℃, electronic energy of 70 eV, quadrupole temperature of 150 ℃, auxiliary heating temperature of 290 ℃, and mass charge ratio of 50-600. The GC-MS fingerprints of 14 batches of Cangai volatile oil were established, and the similarity was evaluated by "Similarity Evaluation System of Chromatographic Fingerprint of Traditional Chinese Medicine(2004 edition)". The common peaks were determined, and their chemical composition and structure were analyzed. Combined with NIST11.L database, the components in the sample were matched qualitatively, and the relative content of each component was calculated by area normalization method.RESULTS There were 21 common peaks in the GC-MS fingerprints of 14 batches of Cangai volatile oil, mainly including eugenol, 1,8-eucalyptol, β-caryophyllene, 4-terpineol and α-pinene. The similarities of 14 batches of samples compared with reference fingerprint were 0.981-0.998.CONCLUSION The established GC-MS fingerprint of Cangai volatile oil has strong characteristics and specificity, which can provide reference for the quality evaluation of Cangai volatile oil.