CDK5 与EMT 相关蛋白在头颈部鳞状细胞癌中异常表达的相关性研究

目的探讨头颈部鳞状细胞癌（HNSCC）中细胞周期素依赖蛋白激酶5（CDK5）及上皮间充质转化（EMT）相关蛋白N-钙黏蛋白（N-cadherin）、波形蛋白（Vimentin）及E-钙黏蛋白（E-cadherin）表达，以及CDK5 的表达与患者预后的关系。方法免疫组化方法检测55 例HNSCC 患者癌组织中CDK5 及EMT 相关蛋白的表达情况；分析不同临床病理特征患者CDK5 及EMT 相关蛋白表达的差异，CDK5 与EMT 相关蛋白表达的相关性，以及CDK5 表达与患者生存之间的关系。结果CDK5 高表达率在有淋巴结转移患者中高于无淋巴结转移患者（91.67% vs 30.23%, P < 0.05），在T3~T4 期患者中高于T1~T2 期患者（85% vs 20%, P < 0.05）；有淋巴结转移患者N-cadherin、Vimentin 高表达率高于无淋巴结转移的患者（75.00% vs 6.98%; 91.67% vs 27.91%, 均P < 0.05），E-cadherin 高表达率低于无淋巴结转移的患者（8.33% vs 86.05%, P < 0.05）；CDK5 与N-cadherin、Vimentin 表达呈正相关，与E-cadherin 表达呈负相关（rs 分别为0.512、0.443、-0.363，均P < 0.01）；CDK5 高表达患者3 年生存率低于低表达患者（37.5% vs 87.0%，Logrank χ2=12.678，P < 0.01）。结论CDK5 及EMT 相关蛋白在转移性HNSCC 中异常表达；CDK5 的表达状态对预测 HNSCC 患者的预后有一定价值。

CDK5 and epithelial-mesenchymal transition related proteins are abnormally expressed in head and neck squamous cell carcinoma

Objective To explore the expressions of Cyclin-dependent kinase 5 (CDK5) and Epithelial-Mesenchymal Transition (EMT) related proteins including N-cadherin, Vimentin and E-cadherin in head and neck squamous cell carcinoma (HNSCC), and to determine the relationship between the expression of CDK5 and prognosis. Methods The expression levels of CDK5 and EMT related proteins were evaluated by immunohistochemistry in 55 patients who were diagnosed as HN⁃ SCC. They were also analyzed in different clinical pathological factors. The correlation of CDK5 and EMT related proteins as well as the relationship between the expression of CDK5 and prognosis were also analyzed. Results The expression level of CDK5 was significantly higher in patients with lymph node metastasis than that in patients with non-lymph node metastasis (91.67% vs 30.23%, P < 0.05). It’s also higher in T3-T4 stages than that in T1-T2 stages (85% vs 20%, P < 0.05). The ex⁃ pression levels of N-cadherin and Vimentin were significantly higher in patients with lymph node metastasis than those in patients with non-lymph node metastasis (75.00% vs 6.98%; 91.67% vs 27.91%, all P < 0.05). However, the expression level of E-cadherin was significantly lower in patients with lymph node metastasis (8.33% vs 86.05%, P < 0.05) compared to that in patients without. CDK5 was positively correlated with N-cadherin and Vimentin, but negatively correlated with E-cad⁃ herin (rs=0.512, 0.443, -0.363, all P < 0.01). The 3-year survival rates were significantly lower in patients with high expres⁃ sion of CDK5 (37.5%) than that in patients with low expression of CDK5 (87%, Log-rank χ2=12.678, P < 0.01). Conclusion CDK5 and EMT related proteins were activated abnormally in HNSCC with lymph node metastasis. CDK5 may be a new bio⁃ logical marker for prognosis of HNSCC.