肺癌相关lncRNA在PM2.5处理细胞中的表达

目的 探讨PM_2.5对人支气管上皮BEAS2B细胞肺癌相关lncRNA表达的影响。 方法 磷酸盐缓冲液(PBS)处理人正常支气管上皮细胞(BEAS2B细胞)为对照组,300 μg/ml PM_2.5处理BEAS2B细胞为处理组,48 h后用实时荧光定量聚合酶链式反应(qRT-PCR)检测lncPVT1、lncUCA1、MIR31HG、lncIRAIN、lncCASC2、lncH19和lncSNHG表达的改变。 结果 与对照组相比,处理组细胞lncPVT1、lncUCA1、MIR31HG和lncIRAIN的表达量均为上升,分别上升了0.93、0.45、2.80和2.94倍,差异均有统计学意义(P<0.05),其中lncIRAIN的表达量上升最为明显。lncCASC2、lncH19的表达量下降,分别下降到0.17和0.08倍,差异均有统计学意义(P<0.05),其中lncH19表达量下降较为明显。 结论 PM_2.5能促进MIR31HG、lncPVT1、lncUCA1和lncIRAIN的表达,抑制lncCASC2和lncH19的表达。

Expression of lung cancer related lncRNAS in BEAS2B cells treated with PM2.5

Objective To explore the impact of fine particulate matter (PM_2.5) on the expression of lung cancer-related lncRNAs in human bronchial epithelial cells (BEAS2B). Methods Normal BEAS2B cells treated with phosphate buffer saline (PBS) for 48 hours served as the control group, while BEAS2B cells treated with 300 μg/ml PM_2.5 for 48 hours was considered as the treated group. The changes of expression of lncPVT1, lncUCA1, MIR31HG, lncIRAIN, lncCASC2, lncH19 and lncSNHG were detected by quantitative real-time polymerase chain reaction assay. Results The expression of lncPVT1, lncUCA1, MIR31HG and lncIRAIN in the treated group was up-regulated and increased by 0.93-fold, 0.45-fold, 2.80-fold and 2.94-fold respectively as compared with the control group, with statistically significant differences (P<0.05). And the expression of lncIRAIN was affected most significantly. The expression of lncCASC2and lncH19in the treated group was down-regulated and decreased by 0.17-fold and 0.08-fold respectively as compared with the control group, with statistically significant differences (P<0.05). And the expression of lncH19 was affected most significantly. Conclusions PM_2.5 can enhance the expression of MIR31HG, lncPVT1, lncUCA1 and lncIRAIN, but inhibit the expression of lncCASC2 and lncH19.