ICP-AES法测定葛根芩连汤中微量元素

目的建立原子发射光谱法测定葛根芩连汤(葛根、黄连、黄芩、甘草)中微量元素的方法及探讨煎煮时间对微量元素析出的影响。方法采用HNO3-HClO4(4∶1)作消化液处理样品,用电感耦合等离子体原子发射光谱(ICP-AES)法同时测定葛根芩连汤60,90 min煎液中Ca,Mg,Zn,Fe,Mn,Cu,Sr,Co,Ni,Cd,Pb等11种微量元素的含有量。结果微量元素的检出限为0.001～0.272μg/mL,相对标准偏差为1.3%～3.8%(n=6),加样回收率在97.33%～102.92%。葛根芩连汤中未检测到Co、Ni、Cd和Pb 4种元素,60 min煎液中Ca、Mg、Zn、Fe、Mn、Cu和Sr的含有量分别为473.39、594.04、4.71、40.33、5.40、1.75、4.93μg/g。葛根芩连汤60 min和90 min煎液中微量元素的含有量基本没有差别。结论所建立的方法简便、准确、灵敏度高。从微量元素析出角度而言,葛根芩连汤宜煎煮60 min。

Determination of trace elements in Gegen Qinlian Decoction by ICP-AES

AIM To determine trace element contents in Gegen Qinlian Decoction(Puerariae lobatae Radix,Coptidis Rhizoma,Scutellariae Radix,Glycyrrhizae Radix et Rhizoma) by atomic emission spectrometry,and to study the relationship between boiling time and trace elements precipitation.METHODS The determination of metal elements in Gegen Qinlian Decoction which was boiled for 60 min and 90 min respectively were studied by ICP-AES after HNO3-HClO4(4∶1)digestion.RESULTS The detection limits of this method were 0.001-0.272 μg/mL,and the RSDs(n=6) were 97.33%-102.92% for all elements.The elements of Co,Ni,Cd and Pb were not detected in the decoction.The contents of Ca、Mg、Zn、Fe、Mn、Cu and Sr were 473.39,594.04,4.71,40.33,5.40,1.75,4.93 μg/g respectively in 60 min detection of Gegen Qinlian Decoction.There was no difference in the contents of elements in Gegen Qinlian Decoction between 60 min decoction and 90 min decoction.CONCLUSION The determination method is simple,accurate,and reliable.For the precipitation of trace elements,the best decoction time for Gegen Qinlian Decoction is 60 min.