皮孔葱臭木化学成分的研究

目的对皮孔葱臭木Dysoxylum lenticellatum茎干的化学成分及其细胞毒活性进行研究。方法利用正相硅胶、RP-18柱色谱、葡聚糖凝胶Sephadex LH-20等手段进行分离纯化,根据波谱数据鉴定化合物的结构,采用MTT法进行细胞毒活性测定。结果分离鉴定了12个化合物,分别为β-谷甾醇(1)、豆甾醇(2)、3-oxo-24,25,26,27-tetranortirucall-7-ene-23(21)-lactone(3)、3α-hydroxy-24,25,26,27-tetranortirucall-7-ene-23(21)-lactone(4)、laxiracemosin H(5)、laxiracemosin B(6)、24,25-epoxy-tirucall-7-ene-3,23-dione(7)、丁酰鲸鱼醇(8)、齐墩果酸(9)、蒲公英赛酮(10)、蒲公英赛醇(11)和8-hydroxy-6-methoxy-3-pentylisocoumarin(12)。结论 12个化合物均为首次从该种植物中分离得到,化合物8、10～12为首次从该属植物中分离得到,化合物5、6是甘遂烷型生物碱,化合物3具有明显体外肿瘤细胞生长抑制活性。

Chemical constituents from Dysoxylum lenticellatum

Objective To study the chemical constituents from the stems of Dysoxylum lenticellatum and their cytotoxic activities.Methods Silica gel,RP-18,and Sephadex LH-20 column chromatography techniques were used for separation and purification of the compounds and extensive spectral analysis spectra were employed for structural elucidation.The cytotoxic activity was evaluated by MTT method.Results Twelve compounds were isolated from D.lenticellatum and their structures were identified as β-sitosterol(1),stigmasterol(2),3-oxo-24,25,26,27-tetranortirucall-7-ene-23(21)-lactone(3),3α-hydroxy-24,25,26,27-tetranortirucall-7-ene-23(21)-lactone(4),laxiracemosin H(5),laxiracemosin B(6),24,25-epoxy-tirucall-7-ene-3,23-dione(7),butyrospermol(8),oleanolic acid(9),taraxerone(10),taraxerol(11),and 8-hydroxy-6-methoxy-3-pentylisocoumarin(12).Conclusion All of these compounds are isolated from D.lenticellatum for the first time and the compounds 8,10—12 are reported from the plants in Dysoxylum Bl.for the first time.What’s more,compounds 5 and 6 are tirucallane-type alkaloids and compound 3 shows significant inhibitory activities against human cancer cell lines in vitro.