补肾活血系列方对血管性痴呆小鼠脑海马病理组织学的影响

目的探讨补肾活血方、补肾方、活血方对血管性痴呆小鼠学习记忆能力及脑海马病理组织学的影响。方法采用反复脑缺血再灌注法复制血管性痴呆小鼠模型,以尼莫地平为阳性对照组,观察补肾活血方、补肾方、活血方3组小鼠水迷宫法行为学及脑海马病理组织学的变化。结果与假手术组比较,模型组小鼠学习与记忆能力下降,表现为游全程时间延长,错误次数增加（P〈0.01）,脑海马明显缺血损伤改变;与模型组比较,各治疗组学习成绩和记忆成绩均明显提高（P〈0.05或P〈0.01）,脑海马病理组织学表现明显改善;各治疗组比较,补肾活血组的学习和记忆成绩以及在改善病理组织学方面优于其他治疗组（P〈0.05或P〈0.01）,补肾组和活血组组间差异无统计学意义。结论补肾活血方、补肾方、活血方均可通过减轻脑海马缺血性损伤,改善血管性痴呆小鼠学习与记忆能力,且补肾活血方优于补肾方与活血方。

The Effect of Bushen Huoxue Decoction Series on the Hippocamp Pathohistology in Vascular Dementia Mice

Objective To observe the effect of Bushen decoction,Huoxue decoction and Bushen Huoxue decoction on ability of learning and memory and the hippocamp pathohistology in brain of vascular dementia mouse.Methods The mouse models with vascular dementia were established by cerebral ischemia reperfusion.Mice in sham operation group and model group were given normal saline.Mice in control troup were given nimodipine.Mice in treatment groups were given Bushen decoction,Huoxue decoction and Bushen Huoxue decoction,respectively.The praxiological changes were observed by water maze,and the hippocamp pathohistology of mouse brain were tested.Results Compared with sham operation group,the ability of learning and memory was reduced in model group,which showed extending the time for swimming in whole process,and increasing times of mistakes（P〈0.01）.The hippocamp pathohistology showed ischemic damage change.Compared with model group,the learning and memorizing performance record,and the hippocamp pathohistology were improved in treatment groups（P〈0.05 or P〈0.01）.The learning and memorizing performance record and the hippocamp pathohistology in Bushen Huoxue decoction group were better than that in other treatment group（P〈0.05 or P〈0.01）.There were no different between Bushen decoction group and Huoxue decoction group.Conclusion The Bushen decoction,Huoxue decoction and Bushen Huoxue decoction could improve ischemic damage of the hippocamp pathohistology,and increase the learning and memory capability in vascular dementia mouse.The effect of Bushen Huoxue decoction was better than that of Bushen decoction and Huoxue decoction.