吉林放射事故病人血浆造血活性观察

目的观察病人血浆造血活性，了解其与照后造血功能变化的可能关系。方法以ＭＴＴ法测病人血浆对ＮＦＳ－６０、ＴＦ－１和ＢＥＴ－２细胞因子依赖细胞株在有或无相应细胞因子存在时的吸光度（４９２ｎｍ）。结果患者血清有类似ＧＭ－ＣＳＦ、ＩＬ－３和ＥＰＯ的刺激活性。血浆加入含ｒｈＧ－ＣＳＦ和ＥＰＯ的培养体系中可使ＮＦＳ－６０和ＢＥＴ－２细胞增殖受抑。上述变化与病人外周血白细胞、红细胞及血红蛋白的变化有相关之处。结论吉林辐射事故病人血浆中有粒系和红系造血抑制活性。

Observation on hemopoietic activities of plasma in an accidentally irradiated patient in Jilin

Objective The hemopoietic activities in the patient's plasma were estimated and the possible relationship between these activities and the hemopoietic function after radiation was analysed,Method Using the MTT assay,the influence of patient's plasma on the proliferation of some cytokine-dependent cell lines(such as NFS-60,TF-1 and BET-2 cells)in the presence or absence of their dependent cytokines was observed.Results Some GM-CSF-like,IL-3-like and Epo like activities could be found in the plasma.Meanwhile,the inhibitory effects on the growth of NFS-60 and BET-2 cells significantly increased when the patient's plasma was added into the culture system with rhG-CSF and rhEpo.These changes were consistent with the changes of WBC,RBC and Hb in peripheral blood of the patient.Conclusion Some erythropoietic and granulopoietic inhibitory activities were found in the plasma of an accidentally irradiated patient.