| Notch1过表达对K562细胞增殖及其周期的影响 |
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| 引用本文： | 杨春秀,陈建斌,张树君,李芳菲. Notch1过表达对K562细胞增殖及其周期的影响[J]. 第三军医大学学报, 2011, 33(6): 574-577 |
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| 作者姓名： | 杨春秀 陈建斌 张树君 李芳菲 |
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| 作者单位： | 杨春秀,陈建斌,张树君,Yang Chunxiu,Chen Jianbin,Zhang Shujun(重庆医科大学附属第一医院血液科,重庆,400016);李芳菲,Li Fangfei(重庆医科大学干细胞与组织工程研究室,重庆,400016) |
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| 摘 要： | 目的 研究外源性Notch1过表达对慢性粒细胞白血病细胞株K562增殖和细胞周期的影响.方法 用脂质体将携带Notch1胞内段(ICN1)的质粒转染入K562细胞,倒置相差显微镜观察转染前后K562细胞形态学变化,RT-PCR 和Western blot检测Notch1 mRNA和蛋白的表达,MTT比色法检测细胞增殖,...
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| 关 键 词： | Notch1信号 过表达 K562细胞 细胞周期 |
Exogenous Notch1 overexpression inhibits proliferation of K562 cells by arresting at G_1 phase |
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| Yang Chunxiu,Chen Jianbin,Zhang Shujun,Li Fangfei. Exogenous Notch1 overexpression inhibits proliferation of K562 cells by arresting at G_1 phase[J]. Acta Academiae Medicinae Militaris Tertiae, 2011, 33(6): 574-577 |
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| Authors: | Yang Chunxiu Chen Jianbin Zhang Shujun Li Fangfei |
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| Affiliation: | Yang Chunxiu1,Chen Jianbin1,Zhang Shujun1,Li Fangfei2(1Department of Hematology,First Affiliated Hospital,2Laboratory of Stem Cell and Tissue Engineering,Chongqing Medical University,Chongqing,400016,China) |
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| Abstract: | Objective To study the effects of overexpression of exogenous Notch1 on cell proliferation and cell cycle of human chronic myeloid leukemia cell line K562.Methods Plasmid pcDNA3.1-ICN1 containing exogenous intracellular fragment of Notch1 was transfected in K562 cells with LipofectamineTM 2000.Inverted phase contrast microscope was used to observe the morphology change of K562 cells,RT-PCR and Western blot analysis were used to detect the expression of Notch1 mRNA and protein.MTT assays and flow cytometry w... |
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| Keywords: | Notch1 signal overexpression K562 cells cell cycle |
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