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葡萄糖对大鼠骨髓破骨细胞骨吸收功能的影响
引用本文:孙彦,李兴,朱亦堃. 葡萄糖对大鼠骨髓破骨细胞骨吸收功能的影响[J]. 中华内分泌代谢杂志, 2009, 25(5). DOI: 10.3760/cma.j.issn.1000-6699.2009.05.023
作者姓名:孙彦  李兴  朱亦堃
作者单位:1. 山东万杰医学院医学系诊断教研室,淄博,255213
2. 山西医科大学第二医院内分泌科
摘    要:目的 观察葡萄糖对大鼠骨髓破骨细胞骨吸收功能的影响,探讨糖尿病骨质疏松的发病机制.方法 用巨噬细胞集落刺激因子、RANKL诱导大鼠骨髓单个核细胞分化为破骨细胞,同时给予不同浓度的葡萄糖(0、5.5、15、25 mmol/L)干预,通过观察骨吸收陷窝数量和面积比及破骨细胞膜表面整合索αvβ3(CD61)表达水平分析葡萄糖对破骨细胞骨吸收功能的影响.结果 (1)高糖(25 mmoL/L)组培养7 d骨吸收陷窝数量和面积比与其他3组相比明显增多(P<0.05或P<0.01).(2)高糖组破骨细胞膜表面CD61表达量及平均荧光强度3 d时与其他3组相比均明显上调(P<0.05或P<0.01);5、7 d时与对照组(0 mmol/L)相比明显上调(P<0.05).结论 高糖可增强破骨细胞的骨吸收功能,这可能是糖尿病骨质疏松的发病机制之一.

关 键 词:葡萄糖  破骨细胞  骨吸收  糖尿病骨质疏松

Effect of glucose on bone resorption by osteoclasts derived from rat bone marrow cells
Abstract:Objective To observe the effect of glucose on bone resorption by osteoclasts derived from rat bone marrow cells,and to study the role of glucose in the pathogenesis of diabetic osteoporosis.Methods Mononuclear cells from rat bone marrow were incubated with α-MEM containing macrophage colony stimulating factor(M-CSF) and receptor activator of NF-κB ligand (RANKL).The culture was treated with glucose of different concentrations(0,5.5,15,25 mmol/L).The effect of glucose on bone resorption by osteoclast was studied by analyzing absorptive lacuna and the expression of integrin αvβ3 (CD61).Results (1) The amount and area ratio of bone absorptive lacuna in high glucose(25 mmoL/L)group showed significant difference from the other 3 groups at day 7 (P<0.05 or P<0.01).(2)The expression of C D61 in osteoclast were up-regulated in high glucose group compared with the other 3 groups at day3 (P<0.05 or P<0.01),and showed signifcant difference with the control (0 mmoL/L)group at day 5and 7 (P<0.05).Conclusion High glucose may enhance bone resorption by osteoclasts,suggesting that this may be one of the key pathogenetic factors of diabetic osteoporosis.
Keywords:Glucose  Osteoclast  Bone resorption  Diabetic osteoporosis
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