亲和离心法检测甲胎蛋白异质体在肝癌诊断中的临床应用

目的采用亲和离心管法检测甲胎蛋白异质体（alpha Fetoprotein-L3,AFP-L3）,分析甲胎蛋白异质体AFP-L3在诊断肝癌的临床应用价值。方法选择150例临床检测甲胎蛋白AFP阳性者（≥20 ng/ml）,使用亲和离心管方法检测甲胎蛋白异质体AFP-L3,研究检测甲胎蛋白异质体（AFP-L3）用于临床鉴别肝癌的作用。结果以甲胎蛋白异质体AFP-L3≥10%作为诊断指标,对78例临床确诊为肝癌的患者甲胎蛋白异质体AFP-L3的诊断灵敏度是91.03%（71/78）;在72例临床诊断为其他肝脏疾病的患者甲胎蛋白异质体AFP-L3的诊断特异性达到95.83%（69/72）;甲胎蛋白异质体AFP-L3的符合率为93.33%[（71＋69）/150]。结论新型亲和离心柱法检测甲胎蛋白异质体AFP-L3对良、恶性肝病的鉴别诊断具有重要临床价值。

The detection of alpha-fetoprotein isoforms using with centrifugal column method in diagnosis of hepatocelluar carcinoma

Objective To explore the clinical significance of alpha-fetoprotein-L3%（AFP-L3） with centrifugal column method in the diagnosis of hepatocelluar carcinoma.Methods The serum samples of 150 patients with elevated AFP level（≥20 ng/ml） were detected by chemiluminescence immunoassay for AFP and centrifugal column method for AFP-L3,and the clinical value of AFP-L3 were studied in the differentiation diagnosis of hepatocelluar cancer.Results Taking AFP-L3≥10 ng/ml as the diagnositic criteria,the sensitivity of AFP-L3 was 91.03%（71/78）in 78 hepatocellular carcinoma patients and the specificity of AFP-L3 was 95.83%（69/72）in 72 other liver diseases patients.The diagnostic accuracy was 93.33%[（71＋69）/150].Conclusion Detection of AFP-L3 seemed be of clinical value in benign or malignant liver disease.