金花茶对衰老大鼠脂代谢及肝细胞凋亡的影响

目的 探讨金花茶调节衰老大鼠脂代谢及抗肝凋亡的作用.方法 健康雄性SD大鼠40只随机分为4组.1组为空白对照组,2～4组予D-半乳糖腹腔注射法建立大鼠亚急性衰老模型后,其中2组作为衰老模型组,3、4组再分别以高、低浓度金花茶灌胃干预40天.测大鼠尾静脉血总胆固醇、甘油三酯、高密度脂蛋白和低密度脂蛋白含量,测肝脏组织总胆固醇、甘油三酯含量和Bcl-2、Bax mRNA表达水平.结果 与衰老模型组相比,高、低浓度金花茶干预组大鼠血清高密度脂蛋白浓度和Bcl-2 mRNA显著升高(P＜0.05)；血清总胆固醇、甘油三酯、低密度脂蛋白浓度和肝组织总胆固醇、甘油三酯含量、Bax mRNA表达水平显著降低(P＜0.05).高、低浓度金花茶组间比较,上述指标有统计学差异(P＜0.05).结论 金花茶可调节衰老大鼠的血脂,减少肝脏脂肪堆积,降低肝细胞凋亡水平,以降低动脉粥样硬化发病风险.

Effect of Camellia Chrysantha (Hu) Tuyama on Lipid Metabolism and Hepatic Apoptosis in Aging Rats

AimTo investigate whether the Camellia chrysantha (Hu) Tuyama can improve lipid metabolism and decrease hepatic apoptosis in aging rats.MethodsForty healthy male SD rats were recruited and divided into two groups randomly as follow: group 1 is normal control, group 2 to group 4 was subacute aging model induced by subcutaneous injection with D-galactose.Group 3 and 4 were treated with high/low Camellia chrysantha concentration respectively, meanwhile the group 2 was treated with saline as the control.Assay the levels of serum lipid in tail vein including the triglyceride (TG), total cholesterol (TC), low density lipoprotein cholesterol (LDLC) and high density lipoprotein cholesterol (HDLC).Test liver issue content of TC, TG, and the level of mRNA expression of Bcl-2 and Bax.ResultsCompared with the control group, serum HDLC level and Bcl-2 expression level of the treatment group increased significantly, while serum TC, TG, LDLC content as well as the TC, TG, Bax expression levels associated with the liver tissues declined markedly (P<0.05).These indicators still exhibited significant difference with comparison between high and low Camellia chrysantha (Hu) Tuyama groups (P<0.05).ConclusionsCamellia chrysantha can improve lipid metabolism, reduce fataccumulation in liver which may decrease the hepatic apoptosis in aging rats, to reduce the risk of atherosclerosis.