九项生化试验对弧菌科10种致病菌鉴定的探讨

目前弧菌科(属种)的鉴定,主要依靠生化性状的试验。本文根据Rypka等(1967)报道的选择最佳鉴别性状的推理方法,从众多的生化试验项目中进行统计学分析优选出九项(即精氨酸、赖氨酸、V-P、阿拉伯糖、甘露糖、蔗糖、无盐胨水、6％盐胨水、10％盐胨水等),组成生化鉴定系统与常规生化试验(11～16项)对疑似弧菌科细菌318株进行鉴定,符合率可达100％;试验结果表明,用最少的生化试验项目,达到了对弧菌科临床常见弧菌的鉴定,既节省了时间,又节约了经费。本次试验将九项生化试验培养基制成后分装安瓿,做成生化鉴定药盒,操作简单,使用方便,对基层实验室有推广价值。

A Preliminary Study on Identification of Vibrio (genera, species)by the Nine Kinds of Biochemical Reaction

Nowadays, the biochemical reaction was also the main method for identification of Vibrio. In this paper accor-ding to Rypka's method authors chose out nine from various kinds of biochemical reaction to identificate Vibrio. It included NO3 reduction, lysine decarboxylase, Voges-Proskauer test, D-Arabi-nose, Mannose, Sucrose and Peptone water containing 0%,6%,10% of NaCl. Using the nine reaction to identificate 318 strains of Vibrio-like bacteria and compare with routine method(11-16 kinds) of biochemical reaction,the corresponding rate was 100%. The results showed that the nine reactionsystem may be used for simple identification of Vibrio.