| 高效液相色谱法测定可乐中咖啡因含量 |
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| 引用本文： | 丁瑞芳,张敏娜,朱军. 高效液相色谱法测定可乐中咖啡因含量[J]. 济宁医学院学报, 2012, 35(2): 106-108 |
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| 作者姓名： | 丁瑞芳 张敏娜 朱军 |
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| 作者单位： | 济宁医学院药学院,山东日照,276826 |
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| 摘 要： | 目的用HPLC测定可乐中咖啡因的含量,建立测定可乐中咖啡因含量的高效液相色谱法的最佳工作条件。方法色谱柱采用kromasilC18柱(250mm×4.6mm,5μm);流动相:甲醇-水(40:60);流速:1.0ml/min;检测波长为275nm;进样量20μl;柱温为室温。结果咖啡因与其它杂质均能达到基线分离,其色谱峰形好。咖啡因的浓度在30～70μg/ml时与峰面积成良好的线性关系(y=27498x+67325,r=0.9994),平均加样回收率为100.1%,方法精密度RSD为1.2%(n=6)。结论该方法准确、简便、快速,适合可乐中咖啡因的含量测定。
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| 关 键 词： | 高效液相色谱法 可乐 咖啡因 |
Determination of caffeine in cola by high performance liquid chromatograph |
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| DING Rui-fang , ZHANG Min-na , ZHU Jun. Determination of caffeine in cola by high performance liquid chromatograph[J]. Jounal of Jinning Medical College, 2012, 35(2): 106-108 |
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| Authors: | DING Rui-fang ZHANG Min-na ZHU Jun |
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| Affiliation: | (School of Pharmaceutical Sciences,Jining Medical University,Rizhao 276826) |
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| Abstract: | Objective To determine the determination of caffeine in cola by high performance liquid chromatograph and optimize the HPLC separation conditions.Methods The chromatographic column kromasil C18(250mm×4.6mm,5μm) and Methanol/H2O(40:60) as mobile phase were used.The flow rate was 1.0ml/min and the detective wavelength was 275nm.Injection volume was 20μl.The column temperature was room temperature.Results Caffeine and other peaks were separated well and the peak shape was presented well.There was a good linearity within the range of 30 to 70μg/ml of caffeine.The average recovery was 101.3%.The RSD was 1.15%(n=6).Conclusion This method is accurate,simple and quick.Therefore it is suitable for the determination of caffeine in cola. |
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| Keywords: | High performance liquid chromatograph Cola Caffeine |
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