| 真核表达载体pcDNA3.1（＋）tPA的构建 |
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| 引用本文： | 赵永波 曹国栋. 真核表达载体pcDNA3.1（＋）tPA的构建[J]. 哈尔滨医科大学学报, 1999, 33(2): 107-109 |
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| 作者姓名： | 赵永波 曹国栋 |
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| 作者单位： | 美国匹兹堡大学医学院神经科 |
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| 基金项目： | 国家自然科学基金,哈尔滨市学科后备带头人基金 |
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| 摘 要： | 组织型纤溶酶原激活剂具有高效、特异的溶栓作用，将人ｔＰＡｃＤＮＡ与真核表达载体ｐｃＤＮＡ３．１（＋）重组，构建一种无形成美丽及激活原癌基因之虞的真核表达载体ｐｃＤＮＡ３．１（＋）ｔＰＡ，以探讨其对纤溶活性的影响。
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| 关 键 词： | 真核表达载体 t－PA 基因转移 血栓性疾病 |
Construction of pcDNA3.1( )tPA Expression Vector |
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| Zhao Yongbo et al. Construction of pcDNA3.1( )tPA Expression Vector[J]. Journal of Harbin Medical University, 1999, 33(2): 107-109 |
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| Authors: | Zhao Yongbo et al |
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| Abstract: | A new kind of recombinant vector containing human tissuetype plasminogen activator(tPA)cDNA was constructed for studing the feasibility of enhancing fibrinolytic activity by transplantation of genetic engineering cells.To construct this kind of expression vector,method of molecular cloning was used.Results showed that the recombinant expression vector containing tPA cDNA has a strong fibrinolytic activity.Conclusion suggested that the new kind of recombinant expression vector could serve as new tools and methods for preventing thrombogenic diseases. |
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| Keywords: | tPA Gene transfer Recombinant expression vector |
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