聪圣胶囊对去皮层血管大鼠前脑BDNF及其受体trkB作用的实验研究

本研究采用WISTAR成年大鼠 ,去除大鼠脑皮层血管建立模型 ,免疫组化采用ABC法 ,研究发现模型组大鼠大脑皮质、海马、大细胞基底核中的BDNF(脑源性神经营养因子 )阳性细胞数量显著减少 ,有的BDNF阳性细胞呈空泡状 ,有的呈萎缩状 ,胞体明显缩小 ,扣带皮质、海马CA1区最明显。结果说明去皮层血管后 ,聪圣胶囊可能激活大鼠皮质、海马等处神经元BDNF、trkB及其mRNA的表达 ,通过其旁分泌和自分泌方式作用自身或邻近神经元 ,也可能通过靶源性的方式作用于大细胞基底核的胆碱能神经元 ,以保护皮质、海马、大细胞基底核神经元免遭变性坏死。

Experimental Study of the Effects of Congsheng Capsules on BDNF and trkB in the Forebrain of the Rats with Cortical Devascularization

The rat dementia model was established by devascularizing the left cortex of the adult Wistar rat. It was found that in the model rats, the number of BDNF positive neurons obviously decreased in the cortex and hippocampus; some of the BDNF positive neurons shrank, and the color of the their cytoplasm faded; some of the BDNF positive neurons became vacuolized, and the color of their cytoplasm became pale; and these changes were most clearly seen in the cingulate cortex and the hippocampal CA 1 area. It was seen that in the experiment, Congsheng Capsules could obviously increase the expressions of BDNF and trkB in the cortex, hippocampus and MBN in the model rats.