铁甲草醇提取物抑制二甲基亚硝胺诱导大鼠肝纤维化的实验研究

目的 观察铁甲草醇提取物抑制二甲基亚硝胺诱导大鼠肝纤维化形成的作用效果,并探讨其可能的作用机制.方法 将60只SD雄性大鼠,随机分为6组,每组10只,分别为正常组,模型组,铁甲草醇提取物高、中、低剂量组(12.6,4.2,1.4 g/kg)和秋水仙碱组(0.2 mg/kg),采用二甲基亚硝胺诱导的大鼠肝纤维化模型,在制备模型的同时,铁甲草高、中、低剂量组和秋水仙碱治疗组均灌胃给药,正常组和模型组均予等量生理盐水灌胃,每天1次,4周后处死大鼠,检测大鼠肝功能、血清透明质酸(HA)、层粘蛋白(LN)和Ⅲ型前胶原氨基端肽(PCⅢ)、肝组织羟脯胺酸(Hyp)含量及肝脏病理组织学改变.结果 铁甲草醇提取物各剂量组血清谷丙转氨酶(ALT)水平及铁甲草高剂量组血清谷草转氨酶(AST)水平与模型组比较,具有显著性差异(P<0.05);铁甲草醇提取物各剂量组大鼠肝组织Hyp含量均明显低于模型组,差异具有显著性(P<0.05);铁甲草醇提取物各剂量组大鼠血清HA、LN、PC Ⅲ含量明显低于模型组,具有显著性差异(P<0.05);铁甲草醇提取物各剂量组肝组织纤维化病变明显减轻,与模型组比较,差异具有显著性(P<0.05).结论 铁甲草醇提取物对二甲基亚硝胺诱导的大鼠肝纤维化形成,具有明显的抑制作用,其作用机制可能与保护肝细胞和抑制胶原纤维合成有关.

Inhibitory Effect of Ethanol Extract of Cassia mimosoides Linn. on Dimethylnitrosamine-induced Hepatic Fibrosis in Rats

Objective To observe the inhibitory effect of ethanol extract of Cassia mimosoides Linn. (EE-CML) on dimethylnitrosamine-induced hepatic fibrosis in rats and to explore its therapeutic mechanism. Methods Sixty male SD rats were equally divided into 6 groups: normal group, model group, high-, middle-and low-dose EE-CML groups (at the doses of 12.6 g/kg, 4. 2 g/kg, and 1.4 g/kg respectively) and colchicine (0. 2 mg/kg). Intervention treatment with EE-CML by gastric gavage was carried out in the rats with hepatic fibrosis which was induced by dimethylnitresamine simulta-neously and their effects were compared with the group treated with colchicine. The model group and the normal group were giv-en the same volume of saline once a day. Four weeks after treatment, hepatic function, levels of serum hyaluronic acid (HA), serum laminin (LN), serum aminoterminal propeptide of type Ⅲ procollagen (PCⅢ), hepatic hydroxyproline (Hyp), and histopathological changes in rats were assayed and examined. Results Compared with those in the model group, in the differences of serum alanine aminotransferase (ALT) level in three EE-CML groups and serum aspartate aminotrans-ferase (AST) level in high dose EE-CML group were statistically significant (P < 0. 05) ; hepatic Hyp level and serum HA, LN and PCⅢNP contents in EE-CML groups were significantly lower than those in the model group (P < 0. 05). Hepatic fi-brosis of rats in EE-CML groups alleviated significantly, and the differences compared with that in the model group were sta-tistically significant (P < 0. 05). Conclusion Ethanol extract of Cassia mimosoides shows significant inhibitory effect on the dimethylnitrosamine-induced hepatic fibrosis in rats, and the inhibitory mechanism may be related to the protection on hepatic cells and the inhibition on collagen fiber synthesis.