促性腺激素对离体培养金黄色田鼠卵泡生长的影响

本文利用器官培养方法观察了成年金黄色田鼠排卵前Day 4(间情期)和Day 1(动情前期)的卵巢。按培养液中所含激素不同,分四组体外培养三天,观察卵泡生长的变化。结果培养液内未加激素、加促卵泡刺激素(FSH)和加促黄体生成素(LH)组的标本,卵泡生长受抑制,卵母细胞退化。培养液内同时加有促卵泡刺激素(FSH 3.75微克/毫升)和促黄体生成素(LH 8微克/毫升)的标本,在Day 4(间情期)大卵泡直径明显增大至477微米以上、卵泡生长近似动情前期大卵泡大小;Day 1(动情前期)大卵泡生长仍在动情前期阶段,未见有排卵现象。表明成年金黄色田鼠卵巢在体外培养条件下,能反映FSH和LH对卵泡生长的影响。

EFFECT OF GONADOTROPINS ON FOLLICUL AR DEVELOPMENT IN THE GOLDEN HAMSTER IN VITRO

The purpose of the present study was to examine the gonadotropic control of follicu-lar development in the Diestrus (Day 4) and Proestrus (Day 1) phases of goldenhamsters in organ culture. Explants of ovaries from adult golden hamsters in Diestrus (Day 4) and Proestrus(Day 1)were maintained in plastic dishes containing 80% Earle's synthetic medium, with 20% fetal calf serum and antibiotic. 4 groups of media were prepared: (1) medium(without hormone), (2) medium containing 3.75 μg/ml Porcine Follicle Stimulating Hor-mone (FSH), (3) medium containing 8 μg/ml Equine Luteini zing Hormone (LH), and(4) medium containing 3.75μg/ml FSH+8μg/ml LH. Histological studies showed thatmarked changes occured in the ovary explant after 3 days in vitro. In ovary explantscultured with FSH or LH alone or without gonadotropin hormone, follicular growth wasseverely retarded and the granulosa cells were pyknotic and darkly stained as comparedwith the controls, and the oocyte displayed degenerative changes. The specimens fromDay 4 were stimulated by the addition of FSH plus LH to the medium; the action ofgonadotropin seemed to promote diestrus follicular growth, their diameters reaching morethan 477μm. Since the diameters of diestrus follicles were from 393 to 476μm. andthose of proestrus follicles from 477 to 560μm or>560μm, the size of the follicles inDiestrus after FSH plus LH treatment in vitro was almost the same as that in theProestrus phase. It has thus been shown that both FSH and LH are required for thelarger follicles of the Proestrus specimens. These results indicate that the growth of thehamster follicle in vitro is relevant to the endocrinological state of the animals, and theaddition of gonadotropins to the culture medium influences the follicular growth of theovary explants.