甘露聚糖结合凝集素-A在早期糖尿病大鼠肾脏中的表达及意义

[摘要] 目的　探讨甘露聚糖结合凝集素-A（Mannose-binding Lectin A，MBL-A）在早期糖尿病大鼠肾脏中表达的变化规律及其与糖尿病肾病(DN)发生发展的关系。 方法　实验分为糖尿病模型组和正常对照组。采用链脲佐菌素(STZ)诱导大鼠DN模型,分别于造模成功后2、4、6、8周,用免疫组织化学、TUNEL染色分析和计算机图像分析技术连续多时点观察肾脏MBL-A的表达变化和细胞凋亡情况,并分析两者之间的关系及其与肾脏病理变化指标的相关性。 结果 糖尿病组2、4、6、8周MBL-A蛋白表达均强于对照组,各时点间也有差异；糖尿病组4、6、8周肾小球细胞凋亡指数（AI）均高于对照组, 各时点间差异也有统计学意义。糖尿病组MBL-A与尿蛋白、肾重/体重（KI）正相关；肾小球细胞AI与尿蛋白、KI正相关；MBL-A与细胞AI呈正相关。 结论 MBL-A在早期糖尿病大鼠肾脏中表达上调,以肾小球表达上调为主, 并随病程延长逐渐增强，其表达变化与糖尿病肾脏病变相关。细胞凋亡也参与了糖尿病早期肾脏病变的变化，激活甘露聚糖结合凝集素，两者相互作用，从而促进糖尿病肾病的发展。

The expression and significance of MBL-A in the kidney of rats with early-stage diabetes

Objective To investigate the expression and significance of Mannose-binding Lectin A(MBL-A) in the renal tissuesof early-stage diabetic rats. Methods The Sprague-Dalwley(SD) rats were randomly divided into the diabetic and control groups.Streptozotocin(STZ)-induced diabetic rat model was established in diabetic group.The expression of MBL-A in renal tissue and apoptosis of renal cells were examined by immunohisochemistry and TUNEL assay,respectively,at 2,4,6 and 8 week.MBL-A expression and the renal index o...