人嗜铬细胞瘤组织中转化生长因子α、肿瘤坏死因子α和血管内皮生长因子的表达

目的观察人嗜铬细胞瘤(PHEO)组织和正常肾上腺髓质中转化生长因子α(TGFα)、肿瘤坏死因子α(TNFα)和血管内皮生长因子(VEGF)表达的差异.方法RT-PCR法比较22例经手术及病理证实的PHEO组织及18例意外死亡者(符合医学伦理学要求)的正常肾上腺髓质中TGFα、TNFα和VEGF mRNA表达的差异;免疫组化法比较石蜡包埋的27例PHEO和14例正常肾上腺髓质中TGFα、TNFα和VEGF蛋白表达的差异,并分析其与肿瘤的性质、部位、大小、重量的关系.结果PHEO中TGFα、TNFα mRNA和蛋白表达均高于正常肾上腺髓质.PHEO中VEGF145剪接体的mRNA表达高于正常肾上腺髓质;VEGF121和VEGF165剪接体的mRNA在PHEO和正常肾上腺髓质中的表达差异无显著性.PHEO的内皮细胞和肿瘤细胞VEGF的染色阳性率高于正常肾上腺髓质.肾上腺外PHEO中TGFα、TNFα和VEGF蛋白表达高于肾上腺PHEO.恶性和多发PHEO的TNFα蛋白表达高于良性和单发PHEO.结论PHEO中TGFα、TNFα和VEGF的mRNA和蛋白表达高于正常肾上腺髓质,TGFα、TNFα和VEGF在不同特点的PHEO中的表达不同.

Expression of transforming growth factor alpha, tumor necrosis factor alpha, and vascular endothelial growth factor of human pheochromocytoma tissues

OBJECTIVE: To compare the expressions of transforming growth factor alpha (TGFalpha), tumor necrosis factor alpha (TNFalpha), and vascular endothelial growth factor (VEGF) between pheochromocytoma (PHEO) tissues and normal adrenal medulla tissues. METHODS: The mRNA expressions of TGFalpha, TNFalpha, and VEGF detected by RT-PCR, were compared between 22 PHEO tissues and 18 normal adrenal medulla tissues (according with the principle of medical ethnics). Immunohistochemistry staining was performed on 27 PHEO tissues and 14 normal adrenal medulla tissues. The comparisons of the protein expression of TGFalpha, TNFalpha, and VEGF were analyzed in both of PHEO tissues and normal adrenal medulla tissues. RESULTS: Compared with normal adrenal medulla tissues, the expressions of TGFalpha and TNFalpha mRNA and protein were higher in PHEO tissues, and VEGF145 mRNA expression was also higher in PHEO tissues, while there was no significant difference of the mRNA expression of VEGF121 and VEGF165 between these two tissues. Positive staining rates for VEGF of endothelial cells and tumor cells were higher in PHEO tissues than in normal adrenal medulla tissues. Expressions of the TGFalpha, TNFalpha, and VEGF protein were higher in extra-adrenal PHEO than in adrenal PHEO. The TNFalpha immunohistochemistry staining rate was higher in the malignant or multiple PHEO than in the benign or single PHEO. CONCLUSIONS: The mRNA and protein expressions of TGFalpha, TNFalpha, and VEGF are higher in PHEO tissues than those in normal adrenal medulla tissues. Expressions of these cytokines vary in PHEO with different characteristic.