| MTT法检测多种肿瘤细胞对硒酸酯多糖的敏感性研究 |
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| 引用本文： | 邓丹,凌娜,季宇彬.MTT法检测多种肿瘤细胞对硒酸酯多糖的敏感性研究[J].亚太传统医药,2009,5(10):16-17. |
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| 作者姓名： | 邓丹 凌娜 季宇彬 |
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| 作者单位： | 哈尔滨商业大学,生命科学与环境科学研究中心,黑龙江,哈尔滨,150076;国家教育部抗肿瘤天然药物工程研究中心,黑龙江,哈尔滨,150076 |
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| 摘 要： | 目的:采用MTT法检测多种肿瘤细胞对硒酸酯多糖(KSC)的敏感性。方法:以30、60、120mg/L3种不同浓度的KSC分别处理HepG-2、Bel-7402、MCF-7、SGC-7901、A5495种肿瘤细胞,采用MTT法检测KSC对5种肿瘤细胞的抑制率。结果:KSC对多种肿瘤细胞都有明显的抑制增殖作用,KSC120mg/L作用72h后,对上述5种细胞株的抑制率分别为69.31%、62.24%、53.29%、52.03%、33.62%。结论:KSC对多种肿瘤细胞都有抑制作用,尤其是对肝癌细胞的抑制作用最强,具有治疗肝癌潜在的应用价值。
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| 关 键 词： | MTT法 硒酸酯多糖(KSC) 肿瘤细胞 敏感性 |
Detection the Sensitivity of Several Cancer Cell Lines to Kappa-Selenocarrageenan by MTT Assay |
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| Deng Dan,Ling Na,ji Yubin.Detection the Sensitivity of Several Cancer Cell Lines to Kappa-Selenocarrageenan by MTT Assay[J].Asia-Pacific Traditional Medicine,2009,5(10):16-17. |
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| Authors: | Deng Dan Ling Na ji Yubin |
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| Institution: | 1.Postdoctoral Research Station of Institute of Materia Medica, Life Science and Environment Science Research Center, Harbin University of Commerce, Harbin 150076, China; 2.Engineering Research Center of Natural Anticancer Drugs , Ministry of Education, Harbin 150076, China) |
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| Abstract: | Objective ：To detect the sensitivity of several cancer cell lines to KappaSelenocarrageenan by M＇IT assay.Methods：Five cancer cell lines included HepG-2,Bel-7402,MCF-7,SGC-7901 and A549 were incubated with Kappa-Selenocarrageenan of various concent ration （30mg/L,60mg/L, 120mg/L）.The inhibition rate was examined by MTT test assay.Results：KSC had a significant killing effect on human cancer ceils in vitro.72 hours latter, the inhibition rates of HepG-2,Bel-7402,MCF-7,SGC-7901and A549 with 120 mg/L KSC separately were 69.31% ,62.24% ,53.29% ,52.03% ,33.62%.Conclusion：KSC has a wide range of inhibition of tumor cells, especially for the strongest inhibition of hepatic carcinoma cells.It might have a promising prospect in the treatment for hepatic carcinoma. |
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| Keywords: | MTT assay Kappa-Selenocarrageenan（KSC） Tumor cells Sensitivity |
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