庆大霉素产生菌23-18诱变育种研究

用青霉素、超声波对庆大霉素产生菌棘孢小单抱菌23-18的孢子进行预处理，以增加其通透性，再用钻60进行辐射诱变，结合耐自身代谢产物的筛选，获得数株高产菌株，其中79，167菌株摇瓶发酵数价比亲株23－18提高20％，发酵周期缩短10h。萌种连续传五代，生产能力稳定，其发酵液用高效液相色谱分析，C组分比例符合中国药典要求。

Mutagenesis and screening of gentamicin producer Micromonospora var. 23-18

ABSTRACT: The spores of Gentamicin(GM) producer Micromonospora echinospora var. 23-18 was treated withpenicillin and ultrasounic to increase the permeability of the cell wall. Then the spores were mutated with 60 Co and themutants were screeneed by GM resistant method. As a results, mutant strains 79 and 168 was obtained, their biologicaltiter were increased by 20%, fermentation period were reduced 10h as compared with the parent strain 23-18.