氦氧饱和潜水环境对小鼠血清皮质酮及白介素-2含量的影响

[目的]观察不同深度、不同时间氦氧饱和暴露处理小鼠血清皮质酮及白介素-2（IL-2）含量的变化，探讨饱和潜水环境对免疫功能影响的规律和可能机制。[方法]将30只6～8周龄的昆明种雄性小鼠随机分为5组，分别为对照组，100m氦氧饱和暴露24h组、72h组和400m氦氧饱和暴露24h组、72h组。以酶联免疫吸附试验（ELISA）测定各组小鼠血清皮质酮及IL-2的含量。[结果]100m氨氧混合气暴露24h及72h后小鼠血清皮质酮含量均较对照组升高（P〈0．05），而IL-2含量未见明显变化；400m氨氧饱和暴露24h及72h后小鼠血清皮质酮含量明显升高（P〈0．01），IL-2含量明显降低（P〈0．01），两种深度下暴露72h与24h组之间的皮质酮及IL-2含量差异均无统计学意义（P〉0．05）。[结论]饱和潜水环境可以引起机体的应激反应，它可能是大深度饱和环境致机体免疫功能抑制的一个重要因素。

Effect of Saturation Diving on Serum Corticosterone and IL-2 in Mice

[Objective] To explore the effect of saturation diving on corticosterone and interleukin 2 in mice and to approach the possible mechanisms of these changes.[Methods] 30 Kunming-raced mice were randomly divided into 5 groups:the control group and simulated saturation diving exposure of 100 m 24 h,100 m 72 h,400 m 24 h and 400 m 72 groups,6 mice for each group.The serum corticosterone and interleukin 2 were assayed by ELISA.[Results] There was a tendency of increase of corticosterone after saturation exposure...