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Engulfment of mast cell secretory granules on skin inflammation boosts dendritic cell migration and priming efficiency
Authors:Jan Dudeck  Julia Froebel  Johanna Kotrba  Christian H.K. Lehmann  Diana Dudziak  Stephan Speier  Sergei A. Nedospasov  Burkhart Schraven  Anne Dudeck
Affiliation:1. Institute for Molecular and Clinical Immunology, Medical Faculty, Otto-von-Guericke University Magdeburg, Magdeburg, Germany;2. Health Campus Immunology, Infectiology and Inflammation, Otto-von-Guericke-University, Magdeburg, Germany;3. Department of Dermatology, Laboratory of Dendritic Cell Biology, University Hospital of Erlangen, Friedrich-Alexander-University of Erlangen-Nürnberg, Erlangen, Germany;4. Paul Langerhans Institute Dresden (PLID) of Helmholtz Zentrum München at the University Clinic Carl Gustav Carus of Technische Universität Dresden, Helmholtz Zentrum München, München-Neuherberg, Germany;5. German Center for Diabetes Research (DZD), München-Neuherberg, Germany;6. DFG-Center for Regenerative Therapies Dresden (CRTD), Faculty of Medicine, Technische Universität Dresden, Dresden, Germany;7. Engelhardt Institute of Molecular Biology and Lomonosov Moscow State University, Moscow, Russia
Abstract:
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  • Keywords:Corresponding author: Anne Dudeck, PhD, Institute for Molecular and Clinical Immunology, Medical Faculty, Otto-von-Guericke University Magdeburg, Leipziger Strasse 44, 39120 Magdeburg, Germany.  Mast cells  dendritic cells  adaptive immune response  T-cell response  skin inflammation  AF  Alexa Fluor  BP  Bandpass filter  CFSE  Carboxyfluorescein succinimidyl ester  CHS  Contact hypersensitivity  DC  Dendritic cell  DNFB  2,4-Dinitro-1-fluorobenzene  eGFP  Enhanced green fluorescent protein  FITC  Fluorescein isothiocyanate  GFP  Green fluorescent protein  LN  Lymph node  MC  Mast cell  MCG  Mast cell granule  MFI  Mean fluorescence intensity  OVA  Ovalbumin  PMC  Peritoneal mast cell  TR  Texas Red  WT  Wild-type
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