宫颈脱落细胞p16^INK4A和p57^KIP2

目的：研究p16^INK4A和p57^KIP2在宫颈脱落细胞的表达及其与临床病理指标的关系。方法：采用免疫细胞化学二步法，检测81例不同病变阶段的宫颈脱落细胞标本中p16^INK4A和p57^KIP2的表达。结果：p16^INK4A在ASC—US、低级别上皮内病变（CINⅠ）、高级别上皮内病变（CINⅡ～Ⅲ）和浸润性癌的阳性表达率分别为6．25％（1／16）、58．8％（10／17）、89．2％（33／37）和100．0％（11／11），各组之间的差异有统计学意义，χ^2=38．806，P〈0．001；p57^KIP2在高级别上皮内病变（CINⅡ～Ⅲ）和浸润性癌的阳性表达率为27．1％（13／48），与ASC—US和低级别上皮内病变（CINⅠ）的阳性表达率为69．7％（23／33）比较，差异有统计学意义，χ^2=15．194，P=0．002。结论：p16^INK4A和p57^KIP2在宫颈脱落细胞中的表达与临床病理指标密切相关，p16^INK4A和p57^KIP2可以作为宫颈癌前病变高危人群的筛查指标。

Expressions and clinical significance of p16^INK4A and p^57KIP2 in cervical exfoliated cells

OBJECTIVE： To investigate the expressions and clinical significance of p16^INK4A and p57^KIP2 proteins in cervical exfoliated cells. METHODS： Eighty-one cases of cervical exfoliated epithelial specimens were examined by immunocytochemiscal two stage method for p16^INK4A and p57^KIP2. RESULTS： The positive rates of p16^INK4A in ASC-US, CIN Ⅰ,CIN Ⅱ-Ⅲ and invasive carcinoma were 6. 25% （1/16）, 58.8%（10/17）, 89.2%（33/37） and 100.0%（11/11）, respec tively. The degree of p16^INK4A expression was correlated with the degree of cervical neoplasia, χ^2 = 38. 806, P〈0. 001. p57^KIP2 was overexpressed in Asc-us and CIN Ⅰ 69.7% （23/33）, corn pared with little expressed in CIN Ⅱ- Ⅲ and invasive carcinoma 27.1% （13/48）, χ^2 =15.194, P= 0.002. CONCLUSIONS： p16^INK4A and p57^KIP2 proteins should be regarded as useful bio markers of cervical neoplasia. Both of them may be significant cytologic markers for screening patients at high risk for developing cervical carcinoma.