心可舒对心肌缺血再灌注损伤的保护作用

目的 :探讨心可舒对心肌缺血再灌注损伤的保护作用 ,并初步探讨其作用机制。方法 :19只家兔随机分为 3组 :①假手术组 (SH组 ,n =6 ) :开胸 ,左冠状动脉前降支 (LAD)只穿线不结扎 ,旷置 2 0min ,再观察 6h ;②缺血 再灌注组 (IR组 ,n =7) ;③心可舒处理组 (XKS组 ,n =6 ) :两组均开胸结扎兔LAD 2 0min ,然后松扎 6h。以电镜下心肌超微结构、心率、再灌注心律失常、左室功能、右室血清SOD活性和MDA的含量及心肌组织中SOD、MDA、ATP水平为观察指标。结果 :3组心率均呈进行性下降趋势 ,其中IR组下降幅度最大。XKS组左室内压力上升和下降速率及左室内压峰值均显著高于IR组 (P <0 .0 1) ,血清及心肌组织中SOD活性和心肌组织中ATP含量显著高于IR组 (P <0 .0 1) ,而MDA含量显著低于IR组 (P <0 .0 1) ,再灌注心律失常发生率低于IR组 (P <0 .0 1) ,XKS组心肌超微结构损害较轻。与SH组比较 ,XKS组上述各指标均无显著差异 (P >0 .0 5 )。结论 :心可舒可促进心肌缺血 再灌注损伤心功能的恢复 ,其作用机制可能与清除氧自由基、改善心肌能量代谢等有关。

Protective Effect of Xinkeshu on Myocardial Ischemia and Reperfussion Injury

Objective: To explore the protective effect of Xinkeshu(XKS) on myocardial ischemia and reperfussion injury(MIRI) and its mechanism. Methods: Ninteen rabbits were randomized into three groups: ①sham handle group(SH group,n=6):Didn't ligate the left anterior descending coronary artery after exposing heart and observed 360 minutes; ②Ischemia/Reperfussion group(IR group,n=7) and ③XKS group(n=6):Myocardial ischemia was induced by ligating the for 20 munites. The ligature was untied to bring about reperfussion for 360 minutes. The effects of XKS on the left ventricular function, heart rate, the probability of reperfusion anhythmias and myocardial ultrastructure of rabbits were observed. The SOD activity and MDA in myocardial tissue and blood and ATP in myocardial tissue were also observed. Results: HR of three groups was inclining to decend. And the decendent rang of the IR group was broadest; Compared with IR group, XKS group increased markedly the +dp/dt max and LVSP(all P<0.01); increased the SOD activity and ATP(P<0.01); reduced the MDA largely (P<0.01); improved myocardial ultrastructure at the end of reperfusion; and the probability of reperfusion anhythmias declined(P<0.05). Conclusion: XKS has a protective effect on MIRI. The mechanism may be to clear the oxygen radicals and improve the myocardial engery metabolism.