PS-341对多发性骨髓瘤细胞分泌BAFF与APRIL及迁移能力的影响 |
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引用本文: | 李午平,罗绍凯,李娟,双跃荣. PS-341对多发性骨髓瘤细胞分泌BAFF与APRIL及迁移能力的影响[J]. 航空航天医药, 2010, 21(6): 885-888 |
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作者姓名: | 李午平 罗绍凯 李娟 双跃荣 |
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作者单位: | 1. 江西省肿瘤医院内一科,江西,南昌,330029 2. 中山大学第一附属医院,广东,广州,510080 |
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摘 要: | 目的:为了研究PS-341对多发性骨髓瘤细胞株RPMI8226、KM-3及10例多发性骨髓瘤原代细胞分泌及迁移能力的影响。方法:在体外培养多发性骨髓瘤(MM)细胞,以PS-341进行处理,用RT-PCR方法检测BAFF与APRI及其3个受体BAFF-R、TACI、BCMAL基因的表达,用ELSIA方法检测细胞培养上清中可溶性BAFF与APRIL的浓度变化,以transwell方法检测细胞迁移能力的变化。结果:MM细胞RPMI8226及KM3表达BAFF与APRIL及3个受体BAFF-R、TACI、BCMA,但部分病人不表达BCMA;PS-341可以抑制MM细胞自分泌可溶性BAFF与APRIL;PS-341降低MM细胞迁移能力。结论:PS-341抑制多发性骨髓瘤细胞分泌BAFF与APRIL,并可以抑制多发性骨髓瘤细胞的迁移。
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关 键 词: | PS-341 多发性骨髓瘤 BAFF APRIL 细胞迁移 |
Effect of PS-341 on Multiple Myeloma Cells Migration and Secretion BAFF and APRIL |
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Affiliation: | LI Wu-ping,LUO Shao-kai,LI Juan,et al.(The Department of First Internal Medicine,The Cancer Hospital of Jiangxi Province,Nanchang 330029,China) |
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Abstract: | Objective:To research the effect of PS-341 on the secretion BAFF and APRIL and cell migration of multiple myeloma cell line RPMI8226 and KM-3 and ten patient's primary multiple myeloma cells.Methods:Cultured these cell in vitro,used RT-PCR detect the changing of mRNA of BAFF and APRIL and their three receptors BAFF R,TACI,BCMA before and after treatment with PS-341;Used ELSIA detect the concentration of BAFF and APRIL in the Supernatants before and after treatment with PS-34;used transwell detect the changing of cell migration ability.Results:Multiple myeloma cell line and ten primary multiple myeloma cell expression APRIL and BAFF and their three receptors TACI,BCMA and BAFF-R,but some of patient's cell don't expression BCMA;PS-341 can inhibit multiple myeloma cell secreted BAFF and APRIL,also inhibited cell migrated.Conclusions:PS-341 can inhibit multiple myeloma cell secreted BAFF and APRIL,also inhibited cell migrated. |
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Keywords: | PS-341 BAFF APRIL |
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