补肾益气活血方对实验性膝骨关节炎兔关节软骨组织形态学的影响

背景目前治疗膝骨关节炎的药物主要是非类固醇类药,应用后可产生胃肠道副作用.研究表明,补肾益气活血方能明显改善膝骨关节炎患者关节疼痛和功能,并且副作用较小.目的通过建立兔膝关节骨关节炎模型,探讨补肾益气活血方对兔病变膝关节软骨病理过程的影响.设计以实验动物为研究对象,随机对照观察研究.单位一所大学动物实验中心.材料健康雄性新西兰兔36只,体质量2.0～3.0kg.方法实验于2002-01/2003-01在广州中医药大学动物实验中心完成.新西兰兔36只,建立Hulth膝骨关节炎模型,术后随机分成3组模型组、醋氨酚组和补肾益气活血方组.各组采取相应的处理方法,术后4,8和12周取材,进行组织病理学评估.主要观察指标主要结局各组关节软骨病理改变积分.次要结局①各组关节软骨肉眼观察.②各组关节软骨光镜观察.结果模型组、醋氨酚组、补肾益气活血方组关节软骨病理变化总积4周分别为(18.50±1.00),(14.25±1.26),(11.75±2.22)分;12周分别为(30.75±1.26),(22.00±3.16),(17.75±2.21)分.各组关节软骨病理变化总积分、软骨细胞病理改变积分和软骨表层病理改变积分依次为模型组＞醋氨酚组＞补肾益气活血方组.组间比较差异均有显著或非常显著性意义.结论补肾益气活血方能延缓膝骨关节炎的病理过程,达到防治膝骨关节炎的作用.

Morphological effect of bushen yiqi huoxue formula on articular cartilage tissue in rabbits with experimental osteoarthritis of knee

BACKGROUND: At present, osteoarthritis of knee is mainly treated with non-steroid drug in medication, but resulting in the side effects on gastrointestinal tract. The research indicates that bushen yiqi huoxue formula, with litter side effect, improves remarkably pain and motor function of patients with osteoarthritis of knee.OBJECTIVE: To probe into the effect of bushen yiqi huoxue formula on pathological process of the cartilage of knee joint by setting up ostoarthritis of knee in rabbt.DESIGN: Randomized controlled study by observation in which the experimental animals were taken as the objects.SETTING: Animal experimental center of a university.MATERIALS: Totally 36 New Zealand normal male rabbits, weighted varied from 2.0 to 3.0 kg.METHODS: The experiment was performed in Animal Experimental Center of Guangzhou University of Chinese Medicine from January 2002 to January 2003. Thirty-six New Zealand rabbits were involved to set up Hulth knee osteoarthritis model. After operation, they were randomized into 3 groups,named as model group, acetaminophen group and bushen yiqi huoxue formula group (formula group). Adopted corresponding managements in each group,the materials were collected at the 4th, 8th and 12th week after operation for histological pathological assess.MAIN OUTCOME MEASURES: Primary results: integrals of pathological changes in cartilage of joint in every group. Secondary results: ①Naked-eye observation of cartilage of joint in every group. ② Optic microscopic observation of cartilage of joint in every group.RESULTS: The total integrals of pathological changes in cartilage of joint in model group, acetaminophen group and bushen yiqi huoxue formula grcup (formula group) at the 4th week were 18.50±1.00, 14.25±1.26,11.75±2.22 respectively, and 30.75±1.26, 22.00±3.16, 17.75±2. 21 at the 12th week respectively. The total integrals of pathological changes in cartilage of joint, in chondrocyte and in cartilage surface layer are in the sequence of model group ＞ acetaminophen group ＞ formula group successively. The significant or very significant differences present in the comparisons between groups.CONCLUSION: Bushen yiqi huoxue formula delays pathological progression of osteoarthritis of knee so that it can treat and prevent the disease.