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| 木香肝毒性组分筛查与GC-MS分析研究 | |
| 引用本文： | 赵筱萍,陆琳,胡斌,王书芳.木香肝毒性组分筛查与GC-MS分析研究[J].浙江大学学报(医学版),2012,41(1):43-46. |
| 作者姓名： | 赵筱萍 陆琳 胡斌 王书芳 |
| 作者单位： | 1. 天津中医药大学,天津300193;浙江中医药大学,浙江杭州310053 2. 浙江中医药大学,浙江杭州,310053 3. 浙江大学药学院,浙江杭州,310058 |
| 摘 要： | 目的:筛查中药材木香中潜在的肝毒性组分。方法:对木香25个化学组分,采用二乙酸荧光素荧光标记法在HepG2细胞模型上筛查肝毒性组分,并使用气相色谱-质谱联用仪分析其化学组成。结果:从木香中筛查出10个肝毒性组分,经气相色谱-质谱联用定性分析,C09组分的主要成分为去氢木香内酯、santamarine(或magnolialide)和reynosin,而C11组分的主要成分是α-木香醇和榄香醇。结论:木香所含的去氢木香内酯、santamarine(或magnolialide)、reynosin、α-木香醇和榄香醇可能具有肝毒性。 |
| 关 键 词： | 气相色谱-质谱法 油类 挥发/分析 木香/分析 内酯类/分析 染色与标记 荧光素类 肝炎 中毒性 |
Screening and identifying hepatotoxic components in Aucklandiae Radix with GC-MS |
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| ZHAO Xiao-ping , LU Lin , HU Bin , WANG Shu-fang.Screening and identifying hepatotoxic components in Aucklandiae Radix with GC-MS[J].Journal of Zhejiang University(Medical Sciences),2012,41(1):43-46. | |
| Authors: | ZHAO Xiao-ping LU Lin HU Bin WANG Shu-fang |
| Institution: | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China. zhaoxiaoping8686@sina.com |
| Abstract: | Objective: To screen the potential hepatotoxic components in Chinese herb medicine Aucklandiae Radix. Methods: The potential hepatotoxic components were screened using HepG2 cells labeled with fluorescein diacetate from 25 fractions of Aucklandiae Radix,in which the hepatotoxic compounds were further identified with GC-MS. Results: Ten potential hepatotoxic fractions were screened.The identification results by GC-MS indicated that the main compounds in C09 were dehydrocostuslactone,santamarine(or magnolialide) and reynosin,and in C11 were α-costol and elemol. Conclusion: Dehydrocostuslactone,santamarine(or magnolialide),reynosin,α-costol and elemol are potential hepatotoxic compounds in Aucklandiae Radix. |
| Keywords: | Gas chromatography-mass spectrometry Oils volatile/analysis Aucklandiae Radix/analysis Lactones/analysis Staining and labeling Fluoresceins Hepatitis toxic |
| 本文献已被 CNKI 万方数据 PubMed 等数据库收录！ | |