Induction of gastric cancer cell adhesion through transforming growth factor-beta1-mediated peritoneal fibrosis |
| |
| Authors: | Zhi-Dong Lv Di Na Fu-Nan Liu Zong-Min Du Zhe Sun Zhen Li Xiao-Yang Ma Zhen-Ning Wang Hui-Mian Xu |
| |
| Affiliation: | 1.Department of Surgical Oncology, The First Hospital of China Medical University, Shenyang 110001, Liaoning Province, China;2.Department of Surgical Oncology, General Hospital of Benxi Iron and Steel, Benxi 117000, Liaoning Province, China;3.Department of General Surgery, The Fourth Hospital of China Medical University, Shenyang 110032, Liaoning Province, China |
| |
| Abstract: | BackgroundPeritoneal dissemination is one of the main causes of death in gastric cancer patients. Transforming growth factor-beta1 (TGF-β1), one of the most potent fibrotic stimuli for mesothelial cells, may play a key role in this processing. The purpose of this study is to elucidate the effects of TGF-β1 on regulation of gastric cancer adhesion to mesothelial cells.MethodsPeritoneal tissues and peritoneal wash fluid were obtained for hematoxylin and eosin staining or ELISA to measure fibrosis and TGF-β1 levels, respectively. The peritoneal mesothelial cell line, HMrSV5, was used to determine the role of TGF-β1 in regulation of gastric cancer cell adhesion to mesothelial cells and expression of collagen, fibronectin, and Smad 2/3 by using adhesion assay, western blot, and RT-PCR.ResultsThe data showed that TGF-β1 treatment was able to induce collagen III and fibronectin expression in the mesothelial cells, which was associated with an increased adhesion ability of gastric cancer cells, but knockdown of minimal sites of cell binding domain of extracellular matrix can partially inhibit these effects.ConclusionPeritoneal fibrosis induced by TGF-β1 may provide a favorable environment for the dissemination of gastric cancer. |
| |
| Keywords: | |
|
|
